Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32831
DC FieldValueLanguage
dc.contributor.authorShiu, J.S.en_US
dc.contributor.author張天傑zh_TW
dc.contributor.authorLiu, S.T.en_US
dc.contributor.authorChang, T.J.en_US
dc.contributor.authorHo, W.C.en_US
dc.contributor.authorLai, S.S.en_US
dc.contributor.authorChang, Y.S.en_US
dc.date1997zh_TW
dc.date.accessioned2014-06-06T07:44:16Z-
dc.date.available2014-06-06T07:44:16Z-
dc.identifier.issn0166-0934zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/32831-
dc.description.abstractE2 is the major neutralizing antigen for classical swine fever virus (CSFV) infection. Previously, we have cloned and sequenced the E2 cDNA of Taiwan strain p97 by the reverse transcription-polymerase chain reaction (RT-PCR) method from CSFV-infected tissue. The presence of RNA splicing donor and acceptor sites were found in the cDNA sequence. In this study, transfection of E2 cDNA into mammalian cells resulted in the production of a spliced RNA. Site-directed mutagenesis of the donor and acceptor sites prevented the RNA splicing event and generated a full length transcript in COS7 cells. Although the spliced E2 transcript has not been reported in natural infection of CSFV, this study suggested that the potential splicing sites affected the E2 gene expression when the plasmid-based E2 gene was introduced into mammalian cells. (C) 1997 Elsevier Science B.V.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Virological Methodsen_US
dc.relation.ispartofseriesJournal of Virological Methods, Volume 69, Issue 1-2, Page(s) 223-230.en_US
dc.relation.urihttp://dx.doi.org/10.1016/s0166-0934(97)00160-2en_US
dc.subjectCSFVen_US
dc.subjectE2 geneen_US
dc.subjectRNA splicingen_US
dc.subjecthog-cholera virusen_US
dc.subjectnucleotide-sequence determinationen_US
dc.subjectmolecular-cloningen_US
dc.subjectpestivirusen_US
dc.subjectglycoproteinen_US
dc.subjectproteinsen_US
dc.subjectgenomeen_US
dc.subjectregionen_US
dc.titleThe presence of RNA splicing signals in the cDNA construct of the E2 gene of classical swine fever virus affected its expressionen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/s0166-0934(97)00160-2zh_TW
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:獸醫學系所
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