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標題: The presence of RNA splicing signals in the cDNA construct of the E2 gene of classical swine fever virus affected its expression
作者: Shiu, J.S.
Liu, S.T.
Chang, T.J.
Ho, W.C.
Lai, S.S.
Chang, Y.S.
關鍵字: CSFV;E2 gene;RNA splicing;hog-cholera virus;nucleotide-sequence determination;molecular-cloning;pestivirus;glycoprotein;proteins;genome;region
Project: Journal of Virological Methods
期刊/報告no:: Journal of Virological Methods, Volume 69, Issue 1-2, Page(s) 223-230.
E2 is the major neutralizing antigen for classical swine fever virus (CSFV) infection. Previously, we have cloned and sequenced the E2 cDNA of Taiwan strain p97 by the reverse transcription-polymerase chain reaction (RT-PCR) method from CSFV-infected tissue. The presence of RNA splicing donor and acceptor sites were found in the cDNA sequence. In this study, transfection of E2 cDNA into mammalian cells resulted in the production of a spliced RNA. Site-directed mutagenesis of the donor and acceptor sites prevented the RNA splicing event and generated a full length transcript in COS7 cells. Although the spliced E2 transcript has not been reported in natural infection of CSFV, this study suggested that the potential splicing sites affected the E2 gene expression when the plasmid-based E2 gene was introduced into mammalian cells. (C) 1997 Elsevier Science B.V.
ISSN: 0166-0934
DOI: 10.1016/s0166-0934(97)00160-2
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