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標題: Cloning and sequencing of full-length cDNA of classical swine fever virus LPC strain
作者: Wong, M.L.
Peng, B.Y.
Liu, J.J.
Chang, T.J.
關鍵字: classical swine fever virus;NS5B;RT-PCR;phylogenetic analysis;hog-cholera virus;nucleocapsid protein;nucleotide-sequence;infectious;rna;pestivirus;genome
Project: Virus Genes
期刊/報告no:: Virus Genes, Volume 23, Issue 2, Page(s) 187-192.
The cDNAs of classical swine fever virus (LPC vaccine strain) were cloned by transcriptase-polymerase chain reaction, and their nucleotide sequences were determined. In this work, we obtained the sequence information of the 786 bases of the 5'-terminal region, 6049 bases of the middle region, and 1648 bases of the 3'-terminal region. Taking our previous results and present data together, the entire genomic sequence of LPC strain was completed (12344 nucleotides in length). The genome of LPC has a large open reading frame that can encode a polypetide of 3897 amino acids, and are flanked by untranslated regions (UTR), 373 bases at the 5'-end and 278 bases at the 3'-end. Phylogenetic analysis based on genomic sequences of several viruses suggested that the LPC strain is closer to Chinese, Riems, HCLV, Alfort/187, Brescia, and Alfort strains in order. After further analysis, we found that an insertion of 13 nucleotides, TTT(C/T)CTTTTTTTT, in the 3'-UTR of LPC, Chinese, and HCLV strains. Immediately downstream to the 13 nucleotides, a unique sequence of LPC consisting of 28 thymidine was observed.
ISSN: 0920-8569
DOI: 10.1023/a:1011856608580
Appears in Collections:獸醫學系所

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