Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32848
DC FieldValueLanguage
dc.contributor.authorChen, S.O.en_US
dc.contributor.author張天傑zh_TW
dc.contributor.authorTsai, T.H.en_US
dc.contributor.authorChang, T.J.en_US
dc.contributor.authorWong, M.L.en_US
dc.contributor.authorSu, H.P.en_US
dc.contributor.authorLiu, J.J.en_US
dc.date2004zh_TW
dc.date.accessioned2014-06-06T07:44:17Z-
dc.date.available2014-06-06T07:44:17Z-
dc.identifier.issn0920-8569zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/32848-
dc.description.abstractThe nucleotide sequence of glycoprotein E of YL vaccine strain was cloned, sequenced and expressed in E. coli. Phylogenetic analysis of envelope ( E) amino acid sequences of 18 JEVs in GenBank showed that the vaccine strain YL closer to the virulent strain HVI which is a Taiwanese isolate. We found only two amino acid mutations (K-138 and G-389) of E protein might lead viral attenuation in YL. In this study, we used pRSET vector system to construct three recombinant plasmids (pRSET/F1R1, pRSET/ F2R2 and pRSET/F1R2), which encoded and expressed different or overlapping amino acid region of E protein. The antigenicity and hemagglutination activity of these recombinant proteins were examined by western blotting and hemagglutination test, respectively. Our results demonstrated that the recombinant protein of pRSET/F1R2 possesses predominant antigenicity and hemagglutination activity.en_US
dc.language.isoen_USzh_TW
dc.relationVirus Genesen_US
dc.relation.ispartofseriesVirus Genes, Volume 28, Issue 2, Page(s) 215-221.en_US
dc.relation.urihttp://dx.doi.org/10.1023/B:VIRU.0000016861.78378.9ben_US
dc.subjectenvelope glycoproteinen_US
dc.subjecthemagglutinationen_US
dc.subjectJapanese encephalitis virusen_US
dc.subjectYLen_US
dc.subjectstrainen_US
dc.subjectphylogenetic analysisen_US
dc.subjectswiss-modelen_US
dc.subjectin-vitroen_US
dc.subjectflavivirusen_US
dc.subjectvirulenceen_US
dc.subjectcloningen_US
dc.subjectmutantsen_US
dc.subjectgenomeen_US
dc.subjectcdnaen_US
dc.subjectmiceen_US
dc.titleExpression of recombinant envelope protein of Japanese encephalitis virus YL strain in Escherichia coli possesses hemagglutination activityen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1023/B:VIRU.0000016861.78378.9bzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
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