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標題: Expression of recombinant envelope protein of Japanese encephalitis virus YL strain in Escherichia coli possesses hemagglutination activity
作者: Chen, S.O.
Tsai, T.H.
Chang, T.J.
Wong, M.L.
Su, H.P.
Liu, J.J.
關鍵字: envelope glycoprotein;hemagglutination;Japanese encephalitis virus;YL;strain;phylogenetic analysis;swiss-model;in-vitro;flavivirus;virulence;cloning;mutants;genome;cdna;mice
Project: Virus Genes
期刊/報告no:: Virus Genes, Volume 28, Issue 2, Page(s) 215-221.
The nucleotide sequence of glycoprotein E of YL vaccine strain was cloned, sequenced and expressed in E. coli. Phylogenetic analysis of envelope ( E) amino acid sequences of 18 JEVs in GenBank showed that the vaccine strain YL closer to the virulent strain HVI which is a Taiwanese isolate. We found only two amino acid mutations (K-138 and G-389) of E protein might lead viral attenuation in YL. In this study, we used pRSET vector system to construct three recombinant plasmids (pRSET/F1R1, pRSET/ F2R2 and pRSET/F1R2), which encoded and expressed different or overlapping amino acid region of E protein. The antigenicity and hemagglutination activity of these recombinant proteins were examined by western blotting and hemagglutination test, respectively. Our results demonstrated that the recombinant protein of pRSET/F1R2 possesses predominant antigenicity and hemagglutination activity.
ISSN: 0920-8569
DOI: 10.1023/B:VIRU.0000016861.78378.9b
Appears in Collections:獸醫學系所

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