Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32850
DC FieldValueLanguage
dc.contributor.authorChen, S.O.en_US
dc.contributor.author張天傑zh_TW
dc.contributor.authorFang, S.H.en_US
dc.contributor.authorShih, D.Y.en_US
dc.contributor.authorChang, T.J.en_US
dc.contributor.authorLiu, J.J.en_US
dc.date2009zh_TW
dc.date.accessioned2014-06-06T07:44:17Z-
dc.date.available2014-06-06T07:44:17Z-
dc.identifier.issn0920-8569zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/32850-
dc.description.abstractNitric oxide (NO) has been shown to suppress Japanese encephalitis virus (JEV) RNA synthesis, viral protein accumulation, and virus release from infected cells. In this article, the potential viral structural proteins as the activators of NO product were studied at the molecular level. First, the genomic region encoding the JEV structural proteins was cloned into a prokaryotic expression vector pET for high-level expression. After purification, these JEV recombinant proteins were added to macrophages to examine the productions of NO and pro-inflammatory mediators. In this study, the recombinant core protein, but not envelope (E), could trigger NO and pro-inflammatory mediators (TNF-alpha, IL-6, and IL-12) productions on macrophages. And their effects were about 85-95% relative to LPS-stimulated macrophages in a dose-dependent manner. Meanwhile, the rCore-2D could up regulate promoters of IL-8 and TNF-alpha via EGFP expression in reporter plasmid (IL-8p-EGFP and TNF-alpha p-EGFP)-transfected cells by flow cytometric analysis. These results suggest that JEV core protein could regulate pro-inflammatory mediators and NO production, and may play a crucial role in the innate immunity for the host to restrict the initial stage of JEV infection.en_US
dc.language.isoen_USzh_TW
dc.relationVirus Genesen_US
dc.relation.ispartofseriesVirus Genes, Volume 38, Issue 1, Page(s) 10-18.en_US
dc.relation.urihttp://dx.doi.org/10.1007/s11262-008-0299-9en_US
dc.subjectJapanese encephalitis virusen_US
dc.subjectCore proteinen_US
dc.subjectNitric oxideen_US
dc.subjecttoll-like receptorsen_US
dc.subjectenvelope proteinen_US
dc.subjectcapsid proteinen_US
dc.subjectnitric-oxideen_US
dc.subjectyl-strainen_US
dc.subjectinfectionen_US
dc.subjectreplicationen_US
dc.subjectlocalizationen_US
dc.subjectexpressionen_US
dc.subjectdeathen_US
dc.titleRecombinant core proteins of Japanese encephalitis virus as activators of the innate immune responseen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1007/s11262-008-0299-9zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
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