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標題: The recombinant nucleocapsid protein of classical swine fever virus can act as a transcriptional regulator
作者: Liu, J.J.
Wong, M.L.
Chang, T.J.
關鍵字: classical swine fever virus;nucleocapsid protein;hog-cholera virus;full-length cdna;nucleotide-sequence;infectious;rna;association;pestivirus;genome
Project: Virus Research
期刊/報告no:: Virus Research, Volume 53, Issue 1, Page(s) 75-80.
The cDNA of the nucleocapsid (core) protein of classical swine fever virus (CSFV) was generated by reverse transcription-polymerase chain reaction (RT-PCR) and cloned into a eukaryotic expression vector. The effect of the recombinant core protein on the transcriptional regulation of cellular as well as viral promoters was studied. Using transient transfection assay, our results demonstrated that the core protein can activate the promoter of human heat shock protein 70 gene, and suppressed the SV40 early promoter. These findings indicate that the core protein appears to function not only as a viral structural protein but also as a regulator of gene expression. The implications of core proteins on the viral maturation are discussed. (C) 1998 Elsevier Science B.V. All rights reserved.
ISSN: 0168-1702
DOI: 10.1016/s0168-1702(97)00132-9
Appears in Collections:獸醫學系所

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