Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32911
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dc.contributor.authorYeh, L.C.en_US
dc.contributor.author陳鵬文zh_TW
dc.contributor.authorLee, W.M.en_US
dc.contributor.authorKoh, B.W.en_US
dc.contributor.authorChan, J.P.en_US
dc.contributor.authorLiu, C.H.en_US
dc.contributor.authorKao, J.P.en_US
dc.contributor.authorChou, C.C.en_US
dc.contributor.author周濟眾zh_TW
dc.contributor.author李衛民zh_TW
dc.date2008zh_TW
dc.date.accessioned2014-06-06T07:44:25Z-
dc.date.available2014-06-06T07:44:25Z-
dc.identifier.issn0032-5791zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/32911-
dc.description.abstractA sensitive ELISA was developed for the detection of amoxicillin (AMX) in serum, urine, and milk. The ELISA used an indirect competitive method produced by coating the plate with ovalbumin conjugated with AMX hapten. Antibodies against AMX-BSA were detected by a goat-antirabbit antibody conjugated with peroxidase. Calibration standard curves ranged from 1.28 ng/mL to 20 mu g/mL [IC50 (inhibition concentration 50%) = 100 ng/mL], and the limits of detection were 1.3, 2.7, and 4.8 ng/mL for urine, milk, and serum, respectively. The intra- and interassay variations were less than 4 and 9.6%. The antibody produced against AMX cross-reacted highly with penicillin G(77%); cross-reacted moderately with ampicillin, oxacillin, and cloxacillin (56.9, 51.4, and 48.8%, respectively); but was considered non-cross-reactive with dicloxacillin (7.4%), cefadroxil (< 1%), and cefazolin (< 1%). Concentrations of AMX were measured simultaneously in venous blood and muscles by using the developed AMX ELISA in an in vivo microdialysis model designed for pigeons. Following i.m. injection (25 mg/kg), AMX attained a peak blood level of 4.74 +/- 0.30 mu g/mL and decreased with a half-life of 2.38 +/- 0.16 h. In contrast, measurements in pectoral and femoral muscles exhibited delayed appearances, reduced peak concentrations, and prolonged half-lives of 4.07 +/- 0.48 (pectoral) and 3.01 +/- 0.26 (femoral) that were significantly different from each other and those in the blood (P < 0.05). Blood protein binding was calculated to be 27.9 +/- 5.7%. This study demonstrated the semiquantitative application of a selective AMX ELISA in the first microdialysis procedure for continuous monitoring of drug levels in specific tissues of pigeons and maybe useful for related studies in other poultry species.en_US
dc.language.isoen_USzh_TW
dc.relationPoultry Scienceen_US
dc.relation.ispartofseriesPoultry Science, Volume 87, Issue 3, Page(s) 577-587.en_US
dc.relation.urihttp://dx.doi.org/10.3382/ps.2007-00167en_US
dc.subjectpigeon microdialysisen_US
dc.subjectamoxicillinen_US
dc.subjectenzyme-linked immunosorbent assayen_US
dc.subjectbeta-lactam antibioticsen_US
dc.subjectin-vivo microdialysisen_US
dc.subjectcolumba-liviaen_US
dc.subjectpenicillin antibioticsen_US
dc.subjectliquid-chromatographyen_US
dc.subjectskeletal-muscleen_US
dc.subjectlung-tissueen_US
dc.subjectpharmacokineticsen_US
dc.subjectplasmaen_US
dc.subjectpenetrationen_US
dc.titleDevelopment of amoxicillin enzyme-linked immunosorbent assay and measurements of tissue amoxicillin concentrations in a pigeon microdialysis modelen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.3382/ps.2007-00167zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
item.grantfulltextnone-
item.fulltextno fulltext-
item.cerifentitytypePublications-
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