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標題: Molecular identification of Anisakis type I larvae isolated from hairtail fish off the coasts of Taiwan and Japan
作者: Umehara, A.
Kawakami, Y.
Ooi, H.K.
Uchida, A.
Ohmae, H.
Sugiyama, H.
關鍵字: Anisakis typica;Anisakis simplex sensu stricto;Anisakis pegreffii;Ribosomal DNA internal transcribed spacer;Hairtail;fragment-length-polymorphism;nuclear ribosomal dna;1845 nematoda;genetic-markers;dujardin;ascaridoidea;systematics;ecology;waters;china
Project: International Journal of Food Microbiology
期刊/報告no:: International Journal of Food Microbiology, Volume 143, Issue 3, Page(s) 161-165.
Anisakid nematodes are known to cause the zoonotic disease, anisakiasis, through the consumption of raw or undercooked fish. The parasites most frequently associated with the disease in humans are categorized as Anisakis type I, which comprise several species of the genus Anisakis. The larvae show primitive forms and lack the detailed morphological characteristics required for precise species identification. Thus, molecular characterization is necessary for determining the species of Anisakis type I larvae and acquiring important clinical and epidemiological information. In this study, we isolated Anisakis type I larvae from hairtail fish caught off the coasts of Taiwan and Japan. The ribosomal DNA (rDNA) internal transcribed spacer (ITS) region was sequenced. and restriction fragment length polymorphism (RFLP) analyses using Hinfl and Hhal was carried out for species identification. Most larvae isolated from hairtail caught in Taiwan were Anisakis typica (84%), while those isolated from hairtail caught in Japan were almost exclusively identified either as Anisakis simplex sensu stricto (65%) or Anisakis pegreffii (33%). This is the first report of A. typica in fish obtained from Taiwan. Our results shed the light on the epidemiology of Anisakis type I larvae, which is a potential cause of human anisakiasis in Taiwan and Japan. (c) 2010 Elsevier B.V. All rights reserved.
ISSN: 0168-1605
DOI: 10.1016/j.ijfoodmicro.2010.08.011
Appears in Collections:獸醫學系所

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