Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/32931
DC FieldValueLanguage
dc.contributor.authorUmehara, A.en_US
dc.contributor.author黃鴻堅zh_TW
dc.contributor.authorKawakami, Y.en_US
dc.contributor.authorOoi, H.K.en_US
dc.contributor.authorUchida, A.en_US
dc.contributor.authorOhmae, H.en_US
dc.contributor.authorSugiyama, H.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-06T07:44:27Z-
dc.date.available2014-06-06T07:44:27Z-
dc.identifier.issn0168-1605zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/32931-
dc.description.abstractAnisakid nematodes are known to cause the zoonotic disease, anisakiasis, through the consumption of raw or undercooked fish. The parasites most frequently associated with the disease in humans are categorized as Anisakis type I, which comprise several species of the genus Anisakis. The larvae show primitive forms and lack the detailed morphological characteristics required for precise species identification. Thus, molecular characterization is necessary for determining the species of Anisakis type I larvae and acquiring important clinical and epidemiological information. In this study, we isolated Anisakis type I larvae from hairtail fish caught off the coasts of Taiwan and Japan. The ribosomal DNA (rDNA) internal transcribed spacer (ITS) region was sequenced. and restriction fragment length polymorphism (RFLP) analyses using Hinfl and Hhal was carried out for species identification. Most larvae isolated from hairtail caught in Taiwan were Anisakis typica (84%), while those isolated from hairtail caught in Japan were almost exclusively identified either as Anisakis simplex sensu stricto (65%) or Anisakis pegreffii (33%). This is the first report of A. typica in fish obtained from Taiwan. Our results shed the light on the epidemiology of Anisakis type I larvae, which is a potential cause of human anisakiasis in Taiwan and Japan. (c) 2010 Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationInternational Journal of Food Microbiologyen_US
dc.relation.ispartofseriesInternational Journal of Food Microbiology, Volume 143, Issue 3, Page(s) 161-165.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.ijfoodmicro.2010.08.011en_US
dc.subjectAnisakis typicaen_US
dc.subjectAnisakis simplex sensu strictoen_US
dc.subjectAnisakis pegreffiien_US
dc.subjectRibosomal DNA internal transcribed spaceren_US
dc.subjectHairtailen_US
dc.subjectfragment-length-polymorphismen_US
dc.subjectnuclear ribosomal dnaen_US
dc.subject1845 nematodaen_US
dc.subjectgenetic-markersen_US
dc.subjectdujardinen_US
dc.subjectascaridoideaen_US
dc.subjectsystematicsen_US
dc.subjectecologyen_US
dc.subjectwatersen_US
dc.subjectchinaen_US
dc.titleMolecular identification of Anisakis type I larvae isolated from hairtail fish off the coasts of Taiwan and Japanen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.ijfoodmicro.2010.08.011zh_TW
item.openairetypeJournal Article-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
Appears in Collections:獸醫學系所
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