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|標題:||Estrous cycle variation of TRPV1-mediated cross-organ sensitization between uterus and NMDA-dependent pelvic-urethra reflex activity||作者:||Peng, H.Y.
|關鍵字:||transient receptor potential vanilloid subfamily member 1;N-methyl-D-aspartate;pelvic pain syndrome;central sensitization;spinal reflex potentiation;capsaicin;spinal cord;postinjury pain hypersensitivity;uterine cervical distension;gene-related peptide;anesthetized rats;urinary-bladder;afferent-fibers;female rat;vanilloid receptor;hypogastric nerve;descending facilitation||Project:||American Journal of Physiology-Endocrinology and Metabolism||期刊/報告no：:||American Journal of Physiology-Endocrinology and Metabolism, Volume 295, Issue 3, Page(s) E559-E568.||摘要:||
Cross-organ sensitization between the uterus and the lower urinary tract (LUT) underlies the high concurrence of pelvic pain syndrome and LUT dysfunctions, and yet the role of gonadal steroids is still unknown. We tested the hypothesis that cross-organ sensitization on pelvic-urethra reflex activity caused by uterine capsaicin instillation is estrous cycle dependent. When compared with the baseline reflex activity (1.00 +/- 0.00 spikes/stimulation), uterine capsaicin instillation significantly increased reflex activity (45.42 +/- 9.13 spikes/stimulation, P < 0.01, n = 7) that was corroborated by an increase in phosphorylated NMDA NR2B (P < 0.05, n = 4) but not NR2A subunit (P > 0.05, n = 4) expression. Both intrauterine pretreatment with capsazepine (5.02 +/- 2.11 spikes/stimulation, P < 0.01, n = 7) and an intrathecal injection of AP5 (3.21 +/- 0.83 spikes/stimulation, P < 0.01, n = 7) abolished the capsaicin-induced cross-organ sensitization and the increment in the phosphorylated NR2B level (P < 0.05, n = 4). The degrees of the cross-organ sensitization increased in a dose-dependent manner with the concentration of instilled capsaicin from 100 to 300 mu M in both the proestrus and metestrus stages, whereas they weakened when the concentrations were higher than 1,000 mu M. Moreover, the cross-organ sensitization caused by the uterine capsaicin instillation increased significantly in the rats during the proestrus stage when compared with the metestrus stage (P < 0.01, n = 7). These results suggest that estrogen levels might modulate the cross-organ sensitization between the uterus and the urethra and underlie the high concurrence of pelvic pain syndrome and LUT dysfunctions.
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