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|標題:||Descending facilitation of spinal NMDA-dependent reflex potentiation from pontine tegmentum in rats||作者:||Chen, G.D.
|關鍵字:||long term potentiation;SRP;pontine tegmentum;serotonin;WAY 100635;8-OH-DPAT;long-term potentiation;external urethral sphincter;lower;urinary-tract;micturition-reflex;anesthetized rats;synaptic-transmission;binding-sites;nerve injury;dorsal-horn;alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid||Project:||American Journal of Physiology-Renal Physiology||期刊/報告no：:||American Journal of Physiology-Renal Physiology, Volume 293, Issue 4, Page(s) F1115-F1122.||摘要:||
This study was conducted to investigate whether dorsolateral pontine tegmentum stimulation modulates spinal reflex potentiation (SRP) and whether serotonergic neurotransmission is involved in such a modulation. Reflex activities of the external urethra sphincter (EUS) electromyogram in response to a test stimulation (TS; 1/30 Hz) or repetitive stimulation (RS; 1 Hz) on the pelvic afferent nerve in 35 anesthetized rats were recorded with/without synchronized train pontine stimulation (PS; 300 Hz, 30 ms) and/or intrathecal administrations of 10 mu l of 2,3-dihydroxy-6-nitro-7-sulfamoyl-benzo (F) quinoxaline (NBQX; 100 mu M), D-2-amino-5-phosphonovalerate (APV; 100 mu M), N-[2-[4-( 2-methoxyphenyl)-1-piperazinyl]ethyl]-N-(2-pyridinyl) cyclohexanecarboxamide trihydrochloride (WAY 100635; 100 mu M), and 8-hydroxy-2-( di-n-propylamino)-tetralin (8-OH-DPAT; 100 mu M). The TS evoked a single action potential (1.00 +/- 0.00 spikes/stimulation), while the RS produced a long-lasting SRP (16.12 +/- 1.59 spikes/stimulation) that was abolished by APV (1.57 +/- 0.29 spikes/stimulation) and was attenuated by NBQX (7.42 +/- 0.57 spikes/stimulation). Synchronized train PS with RS (PS+RS) produced facilitation in RS-induced SRP (25.17 +/- 2.21 spikes/stimulation). Intrathecal WAY 100635 abolished the facilitation in SRP as a result of the synchronized PS (14.66 +/- 1.58 spikes/stimulation). On the other hand, intrathecal 8-OH-DPAT elicited facilitation in the RS-induced SRP (25.16 +/- 1.05 spikes/ stimulation) without synchronized PS. Our findings suggest that dorsolateral pontine tegmentum may modulate N-methyl-D-aspartic acid-dependent SRP via descending serotonergic neurotransmission. This descending modulation may have physiological/pharmacological relevance in the neural controls of urethral closure.
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