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|標題:||Effects of Shugan-Huayu Powder, A Traditional Chinese Medicine, on Hepatic Fibrosis in Rat Model||作者:||Cheng, C.C.
|關鍵字:||liver fibrosis;traditional Chinese herb;hepatic stellate cell;animal;model;sho-saiko-to;dimethylnitrosamine-intoxicated rats;japanese herbal;medicine;prevents liver fibrosis;enzyme-altered lesions;acid-defined;diet;inchin-ko-to;salvia-miltiorrhiza;extracellular-matrix;biliary;obstruction||Project:||Chinese Journal of Physiology||期刊/報告no：:||Chinese Journal of Physiology, Volume 53, Issue 4, Page(s) 223-233.||摘要:||
Shugan-Huayu powder (SHP) has been administered to outpatients with chronic liver disease without clear anti-fibrosis mechanism. To investigate the anti-fibrotic effects of SHP on liver fibrosis in a rat model and in hepatic stellate cells (HSCs) in vitro, rats were gavaged with CCl(4) at 1.0 g/kg body weight twice a week for 8 weeks to induce liver fibrosis and the rats were randomly assigned to one of the three groups: -CCl(4) alone, low-dose SHP and high-dose SHP. SHP was given by gavages 5 times a week for 8 weeks. Serum, livers and HSCs were assayed for serology, pathology, western blot, zy-mography and quantitative RT-PCR. Hepatic function improved as decreased serum aspartate aminotransferase and alanine aminotransferase, and collagen deposition and active HSCs were significantly reduced in CCl(4)-induced liver by SHP treatment. The expression of matrix metalloproteinase-2 (MMP-2) and transforming growth factor-beta 1 (TGF-beta 1) mRNA in fibrotic liver showed significant downregulation after SHP treatment. In vitro, inhibition of a-smooth muscle actin (alpha-SMA) expression and MMP-2 secretion of active HSCs were also noticed by SHP treatment. SHP has an antifibrotic effect on CCl(4)-induced liver fibrosis in rats. Anti-fibrotic mechanisms were probably inhibiting activation of HSCs and decreased expression of MMP-2 and TGF-beta 1.
|Appears in Collections:||獸醫學系所|
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