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標題: Isolation of Trypanosoma (Megatrypanum) theileri from Dairy Cattle in Taiwan
作者: Lee, Y.F.
Cheng, C.C.
Lin, N.N.
Liu, S.A.
Tung, K.C.
Chiu, Y.T.
關鍵字: 18S rDNA;cattle;culture;T. theileri;in-vitro;cell-culture;infection;differentiation;cultivation;sequences;diagnosis;history;rdna;ssu
Project: Journal of Veterinary Medical Science
期刊/報告no:: Journal of Veterinary Medical Science, Volume 72, Issue 4, Page(s) 417-424.
Although Trypanosoma (Megatrypanum) theileri, a blood parasite of bovid species, is spread widely throughout the world. it has never been reported in Taiwan. When an anti-coagulated blood sample from febrile dairy cattle was directly smeared, no parasite was observed. However, a highly distinctive morphological feature of trypanosome appeared in baby hamster kidney (BHK) cell culture in with non-thrown blood bully coat. The different stages and typical ultrastructures of trypanosome were observed in our isolate. The isolate was subsequently identified as T. theileri by species-specific PCR assay (Tth625), 18S rDNA sequencing alignment and internal transcribed spacer of ribosomal genes (ITS) as a marker for molecular phylogenetic analysis. The first T theileri isolate in Taiwan (TWTth1) could be periodically passaged in BHK cell culture for more than one year and retained good re-cryopreservation viability. The BHK culture method would be excellent for diagnostic isolation and maintenance long-term development of this parasite.
ISSN: 0916-7250
Appears in Collections:獸醫學系所

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