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標題: Colon mustard oil instillation induced cross-organ reflex sensitization on the pelvic-urethra reflex activity in rats
作者: Peng, H.Y.
Chen, G.D.
Tung, K.C.
Lai, C.Y.
Hsien, M.C.
Chiu, C.H.
Lu, H.T.
Liao, J.M.
Lee, S.D.
Lin, T.B.
關鍵字: Pelvic Pain;Irritable bowel syndrome;Convergent pathway;Serine;kinase;Glutamate;cyclin-dependent kinase-5;long-term potentiation;postinjury pain;hypersensitivity;nr2b-containing nmda receptors;neuronal-specific;activator;anesthetized rats;dorsal-horn;nr2b subunit;spinal-cord;tyrosine phosphorylation
Project: Pain
期刊/報告no:: Pain, Volume 142, Issue 1-2, Page(s) 75-88.
We investigated the participation of cyclin-dependent kinase-5 (Cdk5)-mediated N-methyl-D-aspartate receptor (NMDAR) NR2B subunit phosphorylation in cross-organ reflex sensitization caused by colon irritation, The external urethral sphincter electromyogram (EUSE) reflex activity evoked by the pelvic afferent nerve test stimulation (TS, I stimulation/30 s) and protein expression in the spinal cord and dorsal root ganglion tissue (T13-L2 and L6-S2 ipsilateral to the Stimulation) in response to colon mustard oil (MO) instillation were tested in anesthetized rats. When compared with a baseline reflex activity with a single action potential evoked by the TS before the administration of test agents, MO instillation into the descending colon sensitized the evoked activity characterized by elongated firing in the reflex activity in association with increased protein levels of Cdk5, PSD95, and phosphorylated NR2B (pNR2B) but not of total NR2B (tNR2B) in the spinal cord tissue. Both cross-organ reflex sensitization and increments in protein expression were reversed fly intra-colonic pretreatments with ruthenium red (a non-selective transient receptor potential vanilloid. TRPV, antagonist), capsaizepine (a TRPV(1)-selective antagonist), lidocaine (a nerve conduction blocker) as well as by the intra-thecal pretreatment with APV (a NRMDR antagonist) Co-101244 (a NR2B-selective antagonist) and roscovitine (a Cdk5 antagonist). Moreover, compared with the control group, both the increase in pNR2B and the cross-organ reflex sensitization were attenuated in the si-RNA of NR2B rats. All these results Suggested that Cdk-dependent NMDAR NR2B Subunit phosphorylation mediates the development of cross-organ pelvic-urethra reflex sensitization Caused by acute colon irritation which could possibly underlie the high concurrence of pelvic pain syndrome with irritable bowel syndrome. (C) 2009 Published by Elsevier B.V. on behalf of International Association for the Study of Pain.
ISSN: 0304-3959
DOI: 10.1016/j.pain.2008.11.017
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