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標題: The N-terminus of porcine circovirus type 2 replication protein is required for nuclear localization and ori binding activities
作者: Lin, W.L.
Chien, M.S.
Du, Y.W.
Wu, P.C.
Huang, C.
關鍵字: Porcine circovirus;Replication protein;DNA binding;Nuclear;localization signal;circle dna-replication;capsid protein;virus;rep;gene
Project: Biochemical and Biophysical Research Communications
期刊/報告no:: Biochemical and Biophysical Research Communications, Volume 379, Issue 4, Page(s) 1066-1071.
Porcine circovirus type 2 possesses a circular, single-stranded DNA genome that requires the replication protein (Rep) for virus replication. To characterize the DNA binding potential and the significant region that confers the nuclear localization of the Rep protein, the defined coding regions of rep gene were cloned and expressed. All of the recombinant proteins except for the N-terminal 110 residues deletion mutant could bind to the double-stranded minimal binding site of replication origin (ori). In addition, the N-terminal deletion mutant lacking 110 residues exhibited mainly cytoplasmic staining in the transfected cells in contrast to the others, which localized dominantly in the nucleus, suggesting that this N-terminal domain is essential for nuclear localization. Furthermore, a series of green fluorescence proteins (GFP) containing potential nuclear localization signal (NLS) sequences were tested for their Cellular distribution. The ability of the utmost 20 residues of the N-terminal region to target the GFP to the nucleus confirmed its role as a functional NLS. (C) 2009 Elsevier Inc. All rights reserved.
ISSN: 0006-291X
DOI: 10.1016/j.bbrc.2009.01.001
Appears in Collections:獸醫病理生物學所

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