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標題: Shikonin inhibits maturation of bone marrow-derived dendritic cells and suppresses allergic airway inflammation in a murine model of asthma
作者: Lee, C.C.
Wang, C.N.
Lai, Y.T.
Kang, J.J.
Liao, J.W.
Chiang, B.L.
Chen, H.C.
Cheng, Y.W.
關鍵字: shikonin;dendritic cells;asthma;allergic inflammation;airway;hyperresponsiveness;mice;antigen;acetylshikonin;protein;derivatives;activation;alkannin;immunity
Project: British Journal of Pharmacology
期刊/報告no:: British Journal of Pharmacology, Volume 161, Issue 7, Page(s) 1496-1511.
BACKGROUND AND PURPOSE Shikonin exhibits a wide range of anti-inflammatory actions. Here, we assessed its effects on maturation of murine bone marrow-derived dendritic cells (BM-DCs) and on allergic reactions in a murine model of asthma. EXPERIMENTAL APPROACH Cultured murine BM-DCs were used to investigate the effects of shikonin on expression of cell surface markers and their stimulation of T-cell proliferation and cytokine production. The therapeutic potential of shikonin was evaluated in a model of allergic airway disease. KEY RESULTS Shikonin dose-dependently inhibited expression of major histocompatibility complex class II, CD80, CD86, CCR7 and OX40L on BM-DCs, induced by a mixture of ovalbumin (OVA; 100 mg.mL(-1)) and thymic stromal lymphopoietin (TSLP; 20 ng.mL(-1)). Shikonin-treated BM-DCs were poor stimulators of CD(4+) T lymphocyte and induced lower levels of interleukin (IL)-4, IL-5, IL-13 and tumour necrosis factor (TNF)-alpha release by responding T-cells. After intratracheal instillation of shikonin in OVA-immunized mice, OVA challenge induced lower IL-4, IL-5, IL-13, TNF-alpha and eotaxin release in bronchial alveolar lavage fluid, lower IL-4 and IL-5 production in lung cells and mediastinal lymph node cells and attenuated OVA-induced lung eosinophilia and airway hyperresponsiveness. CONCLUSION AND IMPLICATIONS Shikonin effectively suppressed OVA + TSLP-induced BM-DC maturation in vitro and inhibited allergic inflammation and airway hyperresponsiveness in a murine model of asthma, showing good potential as a treatment for allergic asthma. Also, our model provides a novel platform for screening drugs for allergic diseases.
ISSN: 0007-1188
DOI: 10.1111/j.1476-5381.2010.00972.x
Appears in Collections:獸醫病理生物學所

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