Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/33091
DC FieldValueLanguage
dc.contributor.authorTsai, S.F.en_US
dc.contributor.author廖俊旺zh_TW
dc.contributor.authorLiu, B.L.en_US
dc.contributor.authorLiao, J.W.en_US
dc.contributor.authorWang, J.S.en_US
dc.contributor.authorHwang, J.S.en_US
dc.contributor.authorWang, S.C.en_US
dc.contributor.authorTzeng, Y.M.en_US
dc.contributor.authorHo, S.P.en_US
dc.contributor.author何素鵬zh_TW
dc.date2003zh_TW
dc.date.accessioned2014-06-06T07:44:53Z-
dc.date.available2014-06-06T07:44:53Z-
dc.identifier.issn0300-483Xzh_TW
dc.identifier.urihttp://hdl.handle.net/11455/33091-
dc.description.abstractThe purpose of this work is to evaluate the pulmonary toxicity of purified thuringiensin in Sprague-Dawley rats. Rats were intratracheally instillated with 0, 0.4, 0.8, 1.6, 3.2, 6.4 and 9.6 mg/kg body weight of thuringiensin. The results indicated that the acute pulmonary LD50 of thuringiensin for rats was 4.4 mg/kg. The total number of inflammatory cells and lactate dehydrogenase (LDH) activity in the bronchoalveolar lavage (BAL) fluid increased in a dose-dependent manner after thuringiensin instillation. Furthermore, an effective dose of 1.6 mg/kg was selected for the time course study of pulmonary toxicity. The treated animals showed a significant increase in the weights of the lungs, hydroxyproline levels in the lungs and total number of cells in BAL fluid 2, 4, 7, 14, 28 and 56 days after treatment. In comparison with the control, the total protein concentrations in BAL fluid were increased by 361, 615, 116, 41, 34 and 41%, after 2, 4, 7, 14, 28 and 56 days, respectively. The LDH activity in BAL fluid showed a significant increase after 1, 2, 4, 7, 14, 28 and 56 days. The increases in fibronectin levels were 164, 552, 490, 769, 335, 257 and 61% at the corresponding times, but neither tumor necrosis factor nor interleukin-1 increased. The treated rats presented abnormal histology including distributed inflammation in the bronchioles and alveoli, bronchial cellular necrosis on days I and 2, and areas of septal thickening with cellular infiltration and collagen deposit in the intestinal and alveolar spaces on days 4-56. Based on these biochemical and pathological parameters, intratracheal instillation of purified thuringiensin might cause significant pulmonary toxicity in rats. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationToxicologyen_US
dc.relation.ispartofseriesToxicology, Volume 186, Issue 3, Page(s) 205-216.en_US
dc.relation.urihttp://dx.doi.org/10.1016/s0300-483x(02)00744-8en_US
dc.subjectthuringiensinen_US
dc.subjectpulmonary toxicityen_US
dc.subjecthydroxyprolineen_US
dc.subjectfibronectinen_US
dc.subjectBacillusen_US
dc.subjectthuringiensisen_US
dc.subjectaeruginosa exotoxin-aen_US
dc.subjectperformance liquid-chromatographyen_US
dc.subjectbronchoalveolar lavage fluiden_US
dc.subjectbacillus-thuringiensisen_US
dc.subjectextracellular-matrixen_US
dc.subjectendothelial-cellsen_US
dc.subjectlung injuryen_US
dc.subjectbleomycinen_US
dc.subjectfibrosisen_US
dc.subjectmiceen_US
dc.titlePulmonary toxicity of thuringiensin administered intratracheally in Sprague-Dawley ratsen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/s0300-483x(02)00744-8zh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.openairetypeJournal Article-
Appears in Collections:獸醫病理生物學所
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