Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/33132
DC FieldValueLanguage
dc.contributor.authorChuang, H.L.en_US
dc.contributor.author陳德勛zh_TW
dc.contributor.authorHuang, Y.T.en_US
dc.contributor.authorChiu, C.C.en_US
dc.contributor.authorChen, H.H.C.en_US
dc.contributor.authorChu, Y.Y.en_US
dc.contributor.authorChen, T.H.en_US
dc.date2009zh_TW
dc.date.accessioned2014-06-06T07:44:58Z-
dc.date.available2014-06-06T07:44:58Z-
dc.identifier.issn0916-7250zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/33132-
dc.description.abstractEpidemiological investigations suggest that increased age is associated with susceptibility to infection. Pseudomonas aeruginosa (P. aeruginosa) infection and associated exotoxin A (PEA) toxicity have been reported in hospitalized elderly patients and young children with cystic fibrosis. The present study investigated age-related differences in PFA-induced hepatotoxicity in post weaning (PW, 3 weeks), young adult (YA, 12 weeks), and mature adult (MA, 60-64 weeks) rats. PEA (20 mu g/kg) was injected intraveneously and mortality, clinical chemistry, hepatic histopathology, TUNEL (Terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling) and PCNA (Proliferating cell nuclear antigen) staining, and serum cytokine levels were assessed at specific time points, up to 72 hr post-exposure (HPE). Mortality in MA rats was 100% at less than 48 HPE. Serum ALT levels in MA rats were approximately 5-fold greater than levels in PW and YA rats at 36 HPE. MA rat liver histological sections showed diffuse hepatocellular necrosis. In contrast. hepatocellular apoptosis, demonstrable by the TUNEL method, was noted simply in the periportal and midzonal regions from 36 to 48 HPE. Increased morphological mitoses and PCNA-positive hepatocytes were seen in PW and YA rats at 72 HPE. These parameters were correlated with age-dependent significant increases in TNF-alpha, IL-2, IL-6. and IL-18 levels. These data suggest that inflammatory cytokines play an important role in age-related differences in PEA-induced hepatotoxicity. Moreover. these cytokines might correlate with different patterns histopathologic features at various ages.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Veterinary Medical Scienceen_US
dc.relation.ispartofseriesJournal of Veterinary Medical Science, Volume 71, Issue 2, Page(s) 163-169.en_US
dc.subjectageen_US
dc.subjectcytokinesen_US
dc.subjectPCNAen_US
dc.subjectPseudomonas aeruginosa exotoxin Aen_US
dc.subjectTUNE Len_US
dc.subjectinduced liver-injuryen_US
dc.subjectkupffer cellsen_US
dc.subjectcytokine productionen_US
dc.subjectin-vitroen_US
dc.subjectt-cellsen_US
dc.subjectmiceen_US
dc.subjectnecrosisen_US
dc.subjectyoungen_US
dc.subjectaccumulationen_US
dc.subjectexpressionen_US
dc.titleInfluence of Age on Susceptibility to Pseudomonas aeruginosa Exotoxin A-induced Hepatotoxicity in Long-Evans Ratsen_US
dc.typeJournal Articlezh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
Appears in Collections:獸醫病理生物學所
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