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標題: The Study of Poly(a-hydroxy) esters Scaffolds Modified by Type II Collagen for Cartilage Repair
作者: 章世豪
Chang, Shih-hao
關鍵字: tissue engineering;組織工程;cartilage repair;polyesters;type II collagen;RGD;scaffolds;軟骨修復;聚酯類高分子;第II型膠原蛋白;RGD;多孔支架
出版社: 化學工程學系
In the study, tissue engineering technique was developed to repair the damage of cartilage. In the experiment, the biodegradable polyesters PLLA and PLGA 50/50, were utilized to fabricate three-dimensional porous scaffolds. Type II collagen and RGD-containing protein were then coated to modify the cell-contacting surface of the scaffolds.
PLLA-PLGA 50/50 blend was found to possess some excellent characters, including good biocompatibility, proper degradation time, strong mechanical strength as well as suitable porosity and pore size. These characteristics are required for tissue engineering scaffolds.
Regarding surface modification, type II collagen coating can promote the growth of chondrocytes, help to maintain their phenotype and increase the production of extracellualr matrix (ECM). RGD modification facilitated cell adhesion, but had no effect on the cell growth rate. Moreover, chondrocytes lost their ability to secrete the chondrocyte-specific matrix.
The advantage of using type II collagen-coated scaffolds was also demonstrated in animal experiments using New Zealand white rabbits. The defect of knee cartilage showed an initial repair after one month, and completely repaired at six month when chondrocytes-seeded collagen coated scaffolds were implanted. We also found the collagen coating-could decrease the degree of inflammation and prevent the migration of fibroblasts into the scaffolds, in a rat subcutaneous model.
In a word, the synthetic PLLA-PLGA 50/50 blend modified by the type II collagen has shown efficacy in repairing the wound of cartilage.

本研究是以組織工程技術來發展軟骨修復的方法。在實驗設計中,我們嘗試以聚酯類高分子(PLLA,PLGA 50/50)製作多孔支架,然後以第II型膠原蛋白及RGD進行材料性質的改質。
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