Please use this identifier to cite or link to this item:
|標題:||Purification of Chitinase Using Peanibacillus sp. Culture Modified Chitin
The affinity chitin chromatography with fermentation-modified chitin was employed for purification of chitinase. For studying chitinase adsorption capacity, factors such as loading temperature, pH and elution solutions were investigated. The optimal loading conditions were found at 15℃, pH5, and elution conditions were: 0.02M acetic acid solution. Under this condition, the obtained specific chitinase activity gave a purification fold of 15.64.
Furthermore, for investigating the mechanism of increase in the purification fold, it was found small particle size of chitin had a better adsorption capacity. Whereas, with the same chitin particle size of mesh 80, it was observed that enzyme-modified chitin gave an increase of 20.6% chitinase adsorption capacity and the fermentation-modified chitin yielded an increase of 54.2% chitinase adsorption capacity. By observing the structural changes of enzyme-modified and fermentation-modified chitin by SEM, it was found the chitin surface became porous & branching structure, which might reveal more active site & reduce the steric hinderance to the adsorption of chitinase.
|Appears in Collections:||化學工程學系所|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.