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|標題:||Evaluation of the alginate culture system for chondrogenesis of human adipose tissue-derived stromal cells and assessment of the rotational speed for the novel bioreactor vessel
|關鍵字:||human adipose tissue derived stromal cell;人類脂肪幹細胞;alginate gel;chondrogenesis;bioreactor;褐藻膠;軟骨新生;生物反應器||出版社:||化學工程學系||摘要:||
In view of that the three-dimensional (3-D) structure was necessary for chondrogenesis, we fabricated alginate beads and pads to be the cell carriers. Human adipose tissue derived stromal cell (ADSC) were embedded in the gel to study the effects of inducers, culture model and components of extracellular matrix on chondrogenesis. Transforming growth factor-beta 3 (TGF-β3) was found to be a better inducer for chondrogenesis of ADSC than TGF-β1. Cell proliferation was limited in divalent cations crosslinked alginate beads; however, matrix synthesis and deposition were benefited from the 3-D environment. Addition of hyaluronic acid in alginate gel increased type II collagen and GAG contents, while addition of chondroitin sulfate promoted cell proliferation and up-regulated the aggrecan gene expression. In the aspect of bioreactor, better results of cell number, scaffold dry weight, mechanical strength and total GAG contents were obtained from dynamic culture than static culture. At the same time, dynamic culture condition stimulated the cells to secrete NO, which could reduce collagen synthesis. Large amount of GAG was released to media upon the flush arising from the rotation. Based on the data of cell proliferation, matrix accumulation and mechanical strength, the optimal rotational speed for the novel bioreactor vessel was smaller than 25 rpm. This study provided a carrier system for chondrogenesis and established suitable parameters for the bioreactor.
|Appears in Collections:||化學工程學系所|
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