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In this study, porous glass fiber membranes were modified with short-chain organosilicon derivatives to prepare the hydrophobic membranes. The organosilicon derivatives tested were octyltriethoxysilane (OTES), butyltrichlorosilane (BTCS), octyldimethylchlorosilane (ODCS), and butyldimethylchlorosilane (BDCS). Based on the elemental analysis (EA) and contact angle measurement, both the immobilized carbon weight and the contact angle results were found in the following order: OTES > BTCS > ODCS > BDCS. The immobilized carbon amount for the OTES membranes was 28.0±9.9 g carbon/cm2, which is about 0.29±0.11 mol OTES/cm2. To investigate the protein adsorption on the modified hydrophobic membranes, batch lysozyme adsorption at 1 M NaCl, 50 mM phosphate bufer, pH 7.45 was conducted. The lysozyme adsorption capacity results are in the order: OTES > BTCS > ODCS > BDCS, same as the EA and contact angle results. It implies that the membranes with higher-degree hydrophobicity could achieve more lysozyme adsorption. The lysozyme adsorption capacity for the OTES membranes was 0.0685±0.0150 mol/cm2, about1.053±0.230 mol/ml, which is larger than that for the C8 gel beads shown in the literature.
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