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dc.contributor.authorChen, Ya-showen_US
dc.description.abstractIn this study, porous glass fiber membranes were modified with short-chain organosilicon derivatives to prepare the hydrophobic membranes. The organosilicon derivatives tested were octyltriethoxysilane (OTES), butyltrichlorosilane (BTCS), octyldimethylchlorosilane (ODCS), and butyldimethylchlorosilane (BDCS). Based on the elemental analysis (EA) and contact angle measurement, both the immobilized carbon weight and the contact angle results were found in the following order: OTES > BTCS > ODCS > BDCS. The immobilized carbon amount for the OTES membranes was 28.0±9.9 g carbon/cm2, which is about 0.29±0.11 mol OTES/cm2. To investigate the protein adsorption on the modified hydrophobic membranes, batch lysozyme adsorption at 1 M NaCl, 50 mM phosphate bufer, pH 7.45 was conducted. The lysozyme adsorption capacity results are in the order: OTES > BTCS > ODCS > BDCS, same as the EA and contact angle results. It implies that the membranes with higher-degree hydrophobicity could achieve more lysozyme adsorption. The lysozyme adsorption capacity for the OTES membranes was 0.0685±0.0150 mol/cm2, about1.053±0.230 mol/ml, which is larger than that for the C8 gel beads shown in the literature.en_US
dc.description.tableofcontents表目錄 表一 改質前後薄膜含碳量的變化 42 表二 改質前後薄膜contact angle值 42 表三 改質前後碳/矽之比例 43 表四 改質後薄膜對lysozyme之批式吸附量(c0 =2.5 mg/ml) 43 表五 Lysozyme isotherm之Langmuir Model參數 44 表六 Lysozyme isotherm之Langmuir-Freundlich Model參數 44 表七 Lysozyme isotherm之Freundlich Model參數 45 表八 改質後薄膜對conalbumin之批式吸附量(c0=2.5 mg/ml) 45 表九 Conalbumin isotherm之Langmuir Model參數 46 表十 Conalbumin isotherm之Langmuir-Freundlich Model參數 46 表十一 Conalbumin isotherms之Freundlich Model參數 47 表十二 改質後薄膜對ovalbumin之批式吸附量(c0 =2.5 mg/ml) 47 表十三 Ovalbumin isotherm之Langmuir Model參數 48 表十四 Ovalbumin isotherm之Langmuir-Freundlich Model參數 48 表十五 Ovnalbumin isotherms之Freundlich Model參數 49 表十六 不同脫附液脫附lysozyme之效果(c0 =2.5 mg/ml) 50 圖目錄 圖一 OTES、BTCS、ODCS、BDCS藥品結構式 51 圖二 OTES及BTCS在薄離纖維薄膜上之反應機構 52 圖三 ODCS及BDCS在薄離纖維薄膜上之反應機構 53 圖四 改質前後之FT-IR圖形 54 圖五 改質後薄膜之contact angle 55 圖六 改質後膜之含碳量 56 圖七 改質前後之C/Si之比值 57 圖八 改質後薄膜對lysozyme之批式吸附量(c0 =2.5 mg/ml) 58 圖九 改質後薄膜對conalbumin之批式吸附量(c0 =2.5 mg/ml) 59 圖十 改質後薄膜對ovalbumin之批式吸附量(c0 =2.5 mg/ml) 60 圖十一 改質後薄膜對lysozyme、conalubmin及ovnalbumin之批式吸附量 (c0=2.5mg/ml) 圖十二 以1 M NaCl, 50 mM phosphate buffer為吸附緩衝溶液下 lysozyme之吸附檢量線 61 圖十三 Lysozyme之吸附等溫線 62 圖十四 以1 M NaCl, 50 mM phosphate buffer為吸附緩衝溶液下conalbumin檢量線 63 圖十五 Conalbumin之吸附等溫線 64 圖十六 以1 M NaCl, 50 mM phosphate buffer為吸附緩衝溶液下ovalbumin檢量線 65 圖十七 Ovalbumin之吸附等溫線 66 圖十八 流動吸附實驗lysozyme之檢量線 67 圖十九 於0.1 mg/ml下 lysozyme之流動吸附實驗結果 68 圖二十 Lysozymey在70 % acetonitrile in DI water之流動脫附實驗之檢量線 69zh_TW
dc.subjectself-assembled monolayeren_US
dc.typeThesis and Dissertationzh_TW
item.openairetypeThesis and Dissertation-
item.fulltextno fulltext-
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