Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/33853
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dc.contributor.authorChen, Chi-Zenen_US
dc.contributor.authorYan, Cheing-Tongen_US
dc.contributor.authorKumar, Ponnusamy Vinothen_US
dc.contributor.authorHuang, Jenn-Wenen_US
dc.contributor.authorJen, Jen-Fonen_US
dc.contributor.other國立中興大學化學系zh_TW
dc.contributor.otherNational Chung Hsing University,Department of Chemistryen_US
dc.date2011-8zh_TW
dc.date.accessioned2014-06-06T07:46:47Z-
dc.date.available2014-06-06T07:46:47Z-
dc.identifier.issn0021-8561zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/33853-
dc.description.abstractIn this study, a simple and novel microdialysis sampling technique incorporating hollow fiber liquid phase microextraction (HF-LPME) coupled online to high-performance liquid chromatography (HPLC) for the one-step sample pretreatment and direct determination of alachlor (2-chloro-2',6'-diethyl-N -(methoxymethyl)acetanilide) and its metabolite 2,6-diethylaniline (2,6-DEA) in microbial culture medium has been developed. A reversed-phase C-18 column was utilized to separate alachlor and 2,6-DEA from other species using an acetonitrile/water mixture (1:1) containing 0.1 M phosphate buffer solution at pH 7.0 as the mobile phase. Detection was carried out with a UV detector operated at 210 nm. Parameters that influenced the enrichment efficiency of online HF-LPME sampling, including the length of the hollow fiber, the perfusion solvent and its flow rate, the pH, and the salt added in sample solution, as well as chromatographic conditions were thoroughly optimized. Under optimal conditions, excellent enrichment efficiency was achieved by the microdialysis of a sample solution (pH 7.0) using hexane as perfusate at the flow rate of 4 mu L/min. Detection limits were 72 and 14 ng/mL for alachlor and 2,6-DEA, respectively. The enrichment factors were 403 and 386 (RSD < 5%) for alachlor and 2,6-DEA, respectively, when extraction was performed by using a 40 cm regenerated cellulose hollow fiber and hexane as perfusion solvent at the flow rate of 0.1 mu L/min. The proposed method provides a sensitive, flexible, fast, and eco-friendly procedure to enrich and determine alachlor and its metabolite (2,6-DEA) in microbial culture medium.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Agricultural and Food Chemistry, Volume 59, Issue 15, Page(s) 8078-8085.en_US
dc.subjectalachloren_US
dc.subject2,6-diethylaniline (2,6-DEA)en_US
dc.subjecthollow fiberen_US
dc.subjectliquid phaseen_US
dc.subjectmicroextractionen_US
dc.subjectHPLC-UVen_US
dc.subjectmicrobial culture mediumen_US
dc.subjectsolid-phase microextractionen_US
dc.subjectionization mass-spectrometryen_US
dc.subjectpriorityen_US
dc.subjectpesticidesen_US
dc.subjectextractionen_US
dc.subjectwateren_US
dc.subjectgasen_US
dc.subjectdegradationen_US
dc.subjectherbicidesen_US
dc.subjecthplcen_US
dc.subjectproductsen_US
dc.titleDetermination of Alachlor and Its Metabolite 2,6-Diethylaniline in Microbial Culture Medium Using Online Microdialysis Enriched-Sampling Coupled to High-Performance Liquid Chromatographyen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1021/jf201129jzh_TW
dc.contributor.catalogerMiao-zhen Luoen_US
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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