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|標題:||Selective voltammetric method for uric acid detection using pre-anodized Nation-coated glassy carbon electrodes||作者:||Zen, J.M.
|關鍵字:||Nafion;uric acid;ascorbic acid;voltammetry;glassy carbon electrode;electrochemical oxidation;surface-properties;paste electrode;pretreatment;adsorption;behavior;sensor;urate||Project:||Analyst||期刊/報告no：:||Analyst, Volume 123, Issue 6, Page(s) 1345-1350.||摘要:||
Electrochemically pre-anodized Nafion-coated glassy carbon electrodes (NCGCEs) were used for the selective determination of uric acid (UA) by square-wave voltammetry. The major interference from ascorbic acid (AA) was overcome owing to the distinct ability of the pre-anodized NCGCE to yield its best performance at around pH 5, At pH 5, AA exists in the anionic form whereas UA is in the cationic form, since their pK(a )values are 4.1 and 5.4, respectively, at 25 degrees C. Consequently, the Nafion film repels the negatively charged AA and selective sensing of UA can be achieved. Moreover, the anodized surface has a high affinity towards UA through hydrogen bonding. Hence the combination of pre-anodization and the Nafion film modification works excellently in the determination of UA. Under optimized conditions, the detection limit for UA has been improved to 10 nM (S/N = 3), The practical analytical utility of the method is demonstrated by the measurement of UA in human urine without any preliminary treatment.
|Appears in Collections:||化學系所|
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