Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/33974
DC FieldValueLanguage
dc.contributor.authorZen, J.M.en_US
dc.contributor.author曾志明zh_TW
dc.contributor.authorChang, M.R.en_US
dc.contributor.authorIlangovan, G.en_US
dc.date1999zh_TW
dc.date.accessioned2014-06-06T07:46:58Z-
dc.date.available2014-06-06T07:46:58Z-
dc.identifier.issn0003-2654zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/33974-
dc.description.abstractSimultaneous determination of guanine and adenine contents in DNA, RNA and synthetic oligonucleotides an a Nafion-ruthenium oxide pyrochlore modified electrode is described. This chemically modified electrode shows very pronounced electrocatalytic effects towards the oxidation of guanine and adenine. The experimental parameters for individual determinations of guanine and adenine on the modified electrode were first optimised. Excellent detection limits (S/N = 3) of 0.86 ng ml(-1) (5.7 nM) and 2.7 ng ml(-1) (20 nM) for guanine and adenine, respectively, were obtained. Simultaneous determination of guanine and adenine in a mixture was also optimised. The denatured calf thymus DNA and yeast RNA showed oxidation peaks corresponding to guanine and adenine with a linear calibration line up to 5 mu g ml(-1) in pH 4.0 phosphate buffer. The detection limit estimated for these nucleic acids were 11.5 ng ml(-1) and 37.7 ng ml(-1) for guanine and adenine, respectively. The new method provides an alternative to estimate the guanine and adenine contents more selectively and sensitively than currently applied techniques.en_US
dc.language.isoen_USzh_TW
dc.relationAnalysten_US
dc.relation.ispartofseriesAnalyst, Volume 124, Issue 5, Page(s) 679-684.en_US
dc.relation.urihttp://dx.doi.org/10.1039/a900532cen_US
dc.subjectperformance liquid-chromatographyen_US
dc.subjectadsorptive stripping voltammetryen_US
dc.subjectcapillary zone electrophoresisen_US
dc.subjectamperometric detectionen_US
dc.subjectnucleic-acidsen_US
dc.subjectcarbon-pasteen_US
dc.subjectpurine-basesen_US
dc.subjectelectrochemical detectionen_US
dc.subjecturic-aciden_US
dc.subjectoxidationen_US
dc.titleSimultaneous determination of guanine and adenine contents in DNA, RNA and synthetic oligonucleotides using a chemically modified electrodeen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1039/a900532czh_TW
item.fulltextno fulltext-
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
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