Please use this identifier to cite or link to this item:
|標題:||Continuous pressurized fluid extraction of Gynostemma pentaphyllum and purification of gypenosides||作者:||Chen, C.H.
|關鍵字:||dammarane-type glycosides;water extraction;carbon-dioxide;saponins;makino||Project:||Industrial & Engineering Chemistry Research||期刊/報告no：:||Industrial & Engineering Chemistry Research, Volume 46, Issue 24, Page(s) 8138-8143.||摘要:||
This work studied extractions of gypenosides from Gynostemma pentaphyllum using, batch and continuous pressurized fluids. Temperature was found to be a dominant factor in extracting gypenosides from this plant material. Using low solid to solvent ratio (50 g/L) was evidenced to avoid a rate limitation on batch pressurized water extraction of gypenosides. Within 3 h of continuous pressurized water extraction at 1.48 MPa, 373 K, 10 mL/min, a high solid to solvent ratio (200 g/L) resulted in 106.7 mg/g gypenosides. Our experimental results showed that 100% recovery of gypenosides, i.e., 164 mg/g, was obtained following pressurized 80% ethanol extraction at the same operation condition. Purification of the extract using Amberlite XAD7-HP column chromatography indicated that the purity of gypenosides increased from 24% to 83% by weight. In the bioassay aspect, the effect of concentrations of gypenosides in the extracts on the growth inhibition of two cancer cells was examined.
|Appears in Collections:||化學系所|
Show full item record
TAIR Related Article
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.