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Development of electroactive species on the immuno-electrochemical detection
|關鍵字:||biological sensor;生物感測器;conductive polymer;electropolymerization;electroactive species;導電高分子;電聚合;電活化物||出版社:||化學工程學系||摘要:||
Electrochemical biosensor is an immunoassay based on the alterations of electrochemical signal. The most significant processes of producing an electrochemical biosensor are labeling protein and immobilizing protein on the gold electrode. In this research, we mainly make use of ascorbic acid modified by K2Cr2O7 to react with HSA. After being modified, ascorbic acid will be labeled to HSA successfully. In protein immobilization, the major explorations are the two methods of physical absorption and electropolymerization entrap protein immobilization, and then compare their performance as well as the processes involved in the prepareation.
Compared with electropolymerization entrap method, the physical absorption one is of more stable results and ease of processes; however, it is rather troublesome that the physical absorption method spend too much time on immobilizing protein, while the electropolymerization entrap method only takes less than 1 minute. If the differences among every electrode can be reduced in the electropolymerization process, this method would still possess some feasibility.
The ampermetric signals could reach 1μA when anti-HSA was physical adsorbed on electrode surface for the immuno responses measurement in a competition mode. And the intra assay deviation could be further reduced to less than 15% which indicates that this approach is worth of further optimization for the potential of practical application.
|Appears in Collections:||化學工程學系所|
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