Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/3403
DC FieldValueLanguage
dc.contributor.advisor劉永銓zh_TW
dc.contributor.advisorKuang-Pin, Hsiungen_US
dc.contributor.advisor熊光濱zh_TW
dc.contributor.author李承璋zh_TW
dc.contributor.authorChang-chung, Leeen_US
dc.date2004zh_TW
dc.date.accessioned2014-06-06T05:31:52Z-
dc.date.available2014-06-06T05:31:52Z-
dc.identifier.urihttp://hdl.handle.net/11455/3403-
dc.description.abstractElectrochemical biosensor is an immunoassay based on the alterations of electrochemical signal. The most significant processes of producing an electrochemical biosensor are labeling protein and immobilizing protein on the gold electrode. In this research, we mainly make use of ascorbic acid modified by K2Cr2O7 to react with HSA. After being modified, ascorbic acid will be labeled to HSA successfully. In protein immobilization, the major explorations are the two methods of physical absorption and electropolymerization entrap protein immobilization, and then compare their performance as well as the processes involved in the prepareation. Compared with electropolymerization entrap method, the physical absorption one is of more stable results and ease of processes; however, it is rather troublesome that the physical absorption method spend too much time on immobilizing protein, while the electropolymerization entrap method only takes less than 1 minute. If the differences among every electrode can be reduced in the electropolymerization process, this method would still possess some feasibility. The ampermetric signals could reach 1μA when anti-HSA was physical adsorbed on electrode surface for the immuno responses measurement in a competition mode. And the intra assay deviation could be further reduced to less than 15% which indicates that this approach is worth of further optimization for the potential of practical application.en_US
dc.description.abstract電化學式生物感測器是一種運用電化學訊號上的變化做為依據的檢測方法,製作電化學生物感測器最主要的程序有,蛋白質的標誌及固定蛋白質於電極上。在本論文中,主要運用經K2Cr2O7改質過的維他命C,與HSA進行反應,達到標誌HSA的目的;在固定蛋白質方面,主要探討物理吸附固定法及電聚合包埋固定法兩種蛋白質固定法,進一步探討電化學生物感測器在免疫分析上之應用。 相較於電聚合包埋固定法,物理吸附固定法結果較佳,製作手續也較為簡便,但因吸附時間過久,對於免疫檢測來說,的確造成相當程度的困擾。而電聚合包埋固定anti-HSA,其包埋時間只需約1分鐘,如果能夠降低因電聚合而產生每片電極之間的差異值,包埋固定仍然具有其可行性。 在物理吸附固定方面,經此類免疫電極,已可將訊號值提高到1μA左右,並且將訊號與誤差值比保持在8:1左右,再經進一步探討,應有實用於針對臨床診斷之潛力。zh_TW
dc.description.tableofcontents摘要 I 英文摘要 II 目錄 III 表目錄 VII 圖目錄 VIII 第一章、緒論 1 第二章、研究背景 3 2-1、生物感測器之原理 3 2-2、生物感測器之組成 3 2-2-1、生物感測元件 3 2-2-2、信號換能器 4 2-2-3、電化學式生物感測器 7 2-3、循環伏安測試法之原理 8 2-4、電極系統的設計 11 2-5、電極表面蛋白質固定法 13 2-5-1、物理性吸附法 13 2-5-2、化學法 14 2-5-3、包埋法 15 2-6、導電高分子簡介 16 2-6-1、導電高分子聚合機制 17 2-6-2、影響polypyrrole電聚合的因素 20 2-7、免疫分析原理及應用 22 2-7-1、抗原 23 2-7-2、抗體 24 2-7-3、抗原-抗體之反應作用 27 2-7-4、免疫分析法 28 2-7-5、電流式免疫感測器 31 2-8、回應曲面法 32 2-8-1、回應曲面法實驗設計方法 32 2-8-2、二水準因子設計 32 2-8-3、陡昇路徑法 34 2-8-4、中心混成設計 35 2-8-5、數據統計分析 36 第三章、實驗 38 3-1、實驗設備 38 3-1-1、電聚合polypyrrol之設備 38 3-1-2、維他命C標誌抗原之設備 38 3-1-3、其他設備 39 3-2、實驗藥品 40 3-3、實驗步驟 41 3-3-1、Pyrrole單體之純化 41 3-3-2清洗電極 42 3-3-3、維他命C(ascorbic acid)之改質 43 3-3-4、改質維他命C與HSA接合反應 44 3-3-5、物理性吸附固定法 46 3-3-5-1、物理吸附之電極片的量測 46 3-3-5-2、添加polylysine以增加改質之維他命C接上HSA之數目 47 3-3-6、以不同方法電聚合polypyrrole 49 3-3-6-1、二極式電極片之包埋法測試 49 3-3-6-2、二極式電聚合過程中加以攪拌 50 3-3-6-3、三極式電極片之包埋法測試 51 3-3-6-4、三極式電聚合過程中加以攪拌 51 3-3-7、電聚合過程中添加pyrrole衍生物之測試 52 3-3-8、競爭型測試 53 第四章、結果與討論 55 4-1、改質維他命C與HSA接合反應之探討 57 4-1-1、二水準因子設計 59 4-1-2、陡昇路徑 61 4-1-3、回應曲面 63 4-2、物理性吸附固定法 68 4-2-1、物理吸附之電極片的量測 69 4-2-2、添加polylysine以增加改質之維他命C接上HSA之數目 70 4-3、電聚合polypyrrole包埋固定 72 4-4、以不同方法電聚合polypyrrole 74 4-5、添加pyrrole衍生物之測試 82 4-6、競爭型測試 83 4-5-1、以物理吸附固定法做競爭型測試 83 4-5-2、以電聚合polypyrrole包埋法做競爭型測試 84 第五章、結論 86 第六章、未來展望 88 參考文獻 89zh_TW
dc.language.isoen_USzh_TW
dc.publisher化學工程學系zh_TW
dc.subjectbiological sensoren_US
dc.subject生物感測器zh_TW
dc.subjectconductive polymeren_US
dc.subjectelectropolymerizationen_US
dc.subjectelectroactive speciesen_US
dc.subject導電高分子zh_TW
dc.subject電聚合zh_TW
dc.subject電活化物zh_TW
dc.title開發電活性物於免疫電化學之檢測上zh_TW
dc.titleDevelopment of electroactive species on the immuno-electrochemical detectionen_US
dc.typeThesis and Dissertationzh_TW
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeThesis and Dissertation-
item.cerifentitytypePublications-
item.fulltextno fulltext-
item.languageiso639-1en_US-
item.grantfulltextnone-
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