Enantioselective synthesis of (S)-2-amino-4-phenylbutanoic acid by the hydantoinase method

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Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/34068

標題:	Enantioselective synthesis of (S)-2-amino-4-phenylbutanoic acid by the hydantoinase method
作者:	Lo, H.H. 李東昇 Kao, C.H. Lee, D.S. Yang, T.K. Hsu, W.H.
關鍵字:	5-[2-phenylethyl]-imidazolidine-2,4-dione;(S)-2-amino-4-phenyl-butanoic;acid;hydantoinase;L-N-carbamoylase;HPLC;chiral stationary-phase;liquid-chromatographic separation;newly;isolated bacterium;amino-acids;enzymatic production;inhibitors;resolution;enzymes
Project:	Chirality
期刊/報告no：:	Chirality, Volume 15, Issue 8, Page(s) 699-702.
摘要:	Biosynthesis of (S)-(+)-2-amino-4-phenylbutanoic acid (1) was performed by nonenantioselective hydantoinase and L-N-carbamoylase using racemic 5-[2-phenylethyl]-imidazolidine-2,4-dione (rac-2) as a substrate. The compounds involved in this biocatalysis process could be simultaneously resolved by high-performance liquid chromatography using Chirobiotic T column with a mobile phase of EtOH/H2O = 10/90 at pH 4.2-4.5. To our knowledge, this is the first report of the successful production of I by the combination of recombinant hydantoinase and L-N-carbamoylase. (C) 2003 Wiley-Liss, Inc.
URI:	http://hdl.handle.net/11455/34068
ISSN:	0899-0042
DOI:	10.1002/chir.10281
Appears in Collections:	化學系所

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