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標題: Enantioselective synthesis of (S)-2-amino-4-phenylbutanoic acid by the hydantoinase method
作者: Lo, H.H.
Kao, C.H.
Lee, D.S.
Yang, T.K.
Hsu, W.H.
關鍵字: 5-[2-phenylethyl]-imidazolidine-2,4-dione;(S)-2-amino-4-phenyl-butanoic;acid;hydantoinase;L-N-carbamoylase;HPLC;chiral stationary-phase;liquid-chromatographic separation;newly;isolated bacterium;amino-acids;enzymatic production;inhibitors;resolution;enzymes
Project: Chirality
期刊/報告no:: Chirality, Volume 15, Issue 8, Page(s) 699-702.
Biosynthesis of (S)-(+)-2-amino-4-phenylbutanoic acid (1) was performed by nonenantioselective hydantoinase and L-N-carbamoylase using racemic 5-[2-phenylethyl]-imidazolidine-2,4-dione (rac-2) as a substrate. The compounds involved in this biocatalysis process could be simultaneously resolved by high-performance liquid chromatography using Chirobiotic T column with a mobile phase of EtOH/H2O = 10/90 at pH 4.2-4.5. To our knowledge, this is the first report of the successful production of I by the combination of recombinant hydantoinase and L-N-carbamoylase. (C) 2003 Wiley-Liss, Inc.
ISSN: 0899-0042
DOI: 10.1002/chir.10281
Appears in Collections:化學系所

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