Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/34156
標題: Solvent-enhanced microwave-assisted derivatization following solid-phase extraction combined with gas chromatography-mass spectrometry for determination of amphetamines in urine
作者: Chung, L.W.
李茂榮
Liu, G.J.
Li, Z.G.
Chang, Y.Z.
Lee, M.R.
關鍵字: Amphetamines;GC-MS;Microwave-assisted derivatization;Solvent;Urine;gc-ms;designer drugs;methamphetamine;microextraction;metabolites;opiates;acids;serum;hair
Project: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
期刊/報告no:: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Volume 874, Issue 1-2, Page(s) 115-118.
摘要: 
An approach using microwave-assisted derivatization (MAD) following solid-phase extraction (SPE) combined with gas chromatography-mass spectrometry (GC-MS) was developed to determine amphetamines in urine samples. The parameters affecting the derivatization efficiency - including microwave power and irradiation time - were investigated. Besides, solvent is thought critically important to MAD. Derivatization performance was studied using various solvents and compared with the performance obtained without solvent. Derivatization efficiency was clearly found to be enhanced by the presence of solvent. The highest derivatization efficiencies were obtained in ethyl acetate (EA) under microwave power of 250 W for I min. Calibration curves for all amphetamines were linear over a range from I to 1000 ng/mL, with correlation coefficients above 0.9992. The intra-day and inter-day precision were less than 15%. The applicability of the method was tested by analyzing amphetamine-abusing subjects urine samples. Accordingly, the solvent-enhanced MAD-CC-MS method appears to be adequate for determining amphetamines in urine. (C) 2008 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/34156
ISSN: 1570-0232
DOI: 10.1016/j.jchromb.2008.09.003
Appears in Collections:化學系所

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