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標題: Identification of new minor metabolites of penicillin G in human serum by multiple-stage tandem mass spectrometry
作者: Ho, H.P.
Lee, R.J.
Chen, C.Y.
Wang, S.R.
Li, Z.G.
Lee, M.R.
關鍵字: beta-lactam antibiotics;liquid-chromatography;allergy;acids;plasma
Project: Rapid Communications in Mass Spectrometry
期刊/報告no:: Rapid Communications in Mass Spectrometry, Volume 25, Issue 1, Page(s) 25-32.
Liquid chromatography/mass spectrometry (LC/MS) and liquid chromatography/tandem mass spectrometry (LC/MS/MS) were applied to characterize drug metabolites. Although these two methods have overcome the identification and structural characterization of metabolites analysis, they remain time-consuming processes. In this study, a novel multiple-stage tandem mass spectrometric method (MS(n)) was evaluated for identification and characterization of new minor metabolism profiling of penicillin G, one of the beta-lactam antibiotics, in human serum. Seven minor metabolites including five phase I metabolites and two phase II metabolites of penicillin G were identified by using data-dependent LC/MS(n) screening in one chromatographic run. The accuracy masses of seven identified metabolites of penicillin G were also confirmed by mass spectral calibration software (MassWorks (TM)). The proposed data-dependent LC/MS(n) method is a powerful tool to provide large amounts of the necessary structural information to characterize minor metabolite in metabolism profiling. Copyright (C) 2010 John Wiley & Sons, Ltd.
ISSN: 0951-4198
DOI: 10.1002/rcm.4823
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