Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/34176
標題: Determination of ketamine and metabolites in urine by liquid chromatography-mass spectrometry
作者: Chen, C.Y.
李茂榮
Lee, M.R.
Cheng, F.C.
Wu, G.J.
關鍵字: ketamine;metabolites;liquid chromatography-mass spectrometry;electrospray ionization;atmospheric pressure chemical ionization;solid-phase microextraction;micellar electrokinetic chromatography;amphetamine-type substances;human plasma;electrospray-ionization;chemical derivatization;hair analysis;norketamine;dehydronorketamine;capillary
Project: Talanta
期刊/報告no:: Talanta, Volume 72, Issue 3, Page(s) 1217-1222.
摘要: 
We have developed an analytical method by using liquid chromatography-mass spectrometry (LC-MS) to determine ketamine and its metabolites in urine. The ionization efficiency between two ionization modes (ESI and APCI) of LC-MS was compared to each other. An easy and simple sample preparation of urine samples was made by passing samples through a 0.22 mu m PVDF syringe filter. The results indicated that the ionization efficiency of positive APCI mode is better than positive ESI mode for determination of trace ketamines. A wide linearity range of the research is from 5 to 250 ng mL(-1) and the detection limits for ketamine, norketarnine and dehydronorketamine were 0.95, 0.48 and 0.33 ng mL(-1), respectively. The proposed method was tested by analyzing ketamine and metabolites in the urines of volunteers. The concentrations of ketamine, norketamine and dehydronorketamine are ranged of 5.4-131.0, 12.5-74.1 and 22.8-278.9 ng mL(-1), respectively and the ketamines concentration profiles in human urine were also determined. The results demonstrate the suitability of liquid chromatography-mass spectrometry approach to analyze trace amount of ketamine and its metabolites in urine. (C) 2007 Elsevier B.V. All rights reserved.
URI: http://hdl.handle.net/11455/34176
ISSN: 0039-9140
DOI: 10.1016/j.talanta.2007.01.016
Appears in Collections:化學系所

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