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|標題:||Determination of ketamine and metabolites in urine by liquid chromatography-mass spectrometry||作者:||Chen, C.Y.
|關鍵字:||ketamine;metabolites;liquid chromatography-mass spectrometry;electrospray ionization;atmospheric pressure chemical ionization;solid-phase microextraction;micellar electrokinetic chromatography;amphetamine-type substances;human plasma;electrospray-ionization;chemical derivatization;hair analysis;norketamine;dehydronorketamine;capillary||Project:||Talanta||期刊/報告no：:||Talanta, Volume 72, Issue 3, Page(s) 1217-1222.||摘要:||
We have developed an analytical method by using liquid chromatography-mass spectrometry (LC-MS) to determine ketamine and its metabolites in urine. The ionization efficiency between two ionization modes (ESI and APCI) of LC-MS was compared to each other. An easy and simple sample preparation of urine samples was made by passing samples through a 0.22 mu m PVDF syringe filter. The results indicated that the ionization efficiency of positive APCI mode is better than positive ESI mode for determination of trace ketamines. A wide linearity range of the research is from 5 to 250 ng mL(-1) and the detection limits for ketamine, norketarnine and dehydronorketamine were 0.95, 0.48 and 0.33 ng mL(-1), respectively. The proposed method was tested by analyzing ketamine and metabolites in the urines of volunteers. The concentrations of ketamine, norketamine and dehydronorketamine are ranged of 5.4-131.0, 12.5-74.1 and 22.8-278.9 ng mL(-1), respectively and the ketamines concentration profiles in human urine were also determined. The results demonstrate the suitability of liquid chromatography-mass spectrometry approach to analyze trace amount of ketamine and its metabolites in urine. (C) 2007 Elsevier B.V. All rights reserved.
|Appears in Collections:||化學系所|
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