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標題: High throughput screening various abused drugs and metabolites in urine by liquid chromatography-heated electrospray ionization/tandem mass spectrometry
作者: Chen, C.Y.
Shen, C.C.
Yang, T.J.
Chang, Y.Z.
Lee, M.R.
關鍵字: High-throughput screening;Abused drugs;Heated electrospray ionization;Tandem mass spectrometry;Urine;lc-ms/ms method;oral fluid;phase microextraction;gc-ms;hair;samples;plasma;methamphetamine;identification;optimization
Project: Talanta
期刊/報告no:: Talanta, Volume 77, Issue 4, Page(s) 1351-1357.
An integrated method of liquid chrornatography-heated electrospray ionization/tandem mass spectrometry was evaluated for high throughput screening of various abused drugs in urine. Chromatographic analysis was performed on a C18 reverse phase column using a linear gradient of 10mM ammonium acetate containing 0.1% formic acid-methanol as mobile phase and the total separation time was 7 min. A simple and rapid sample preparation method used was by passing urine samples through a 0.22 mu m PVDF syringe filter. The detection limits of the studied abused drugs in urine were from 0.6 ng mL(-1) (ketamine) to 9.0 ng mL(-1) (norcodeine). According to the results, the linear range was from 1 to 1200 ng mL(-1) with relative standard deviation (R.S.D.s) value below 14.8% (intra-day) and 24.6% (inter-day). The feasibility or applying the proposed method to determine various abused drugs in real samples was examined by analyzing urine samples from drug-abused suspects. The abused drugs including ketamines and amphetamines were detected in suspected urine samples. The results demonstrate the suitability of LC-HESI-MS/MS for high throughput screening of the various abused drugs in urine. (C) 2008 Elsevier B.V. All rights reserved.
ISSN: 0039-9140
DOI: 10.1016/j.talanta.2008.09.017
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