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|標題:||Quality control of Chinese medicinal preparations LC/ESI(+)/MS/MS analyses of saikosaponins-a and -c as markers of Bupleuri radix samples||作者:||Liau, B.C.
|關鍵字:||Bupleuri radixes;Chaihu;saikosaponin-a;saikosaponin-c;LC/ESI(+)/MS/MS;mass-spectrometry;chromatography;saponins||Project:||Journal of Pharmaceutical and Biomedical Analysis||期刊/報告no：:||Journal of Pharmaceutical and Biomedical Analysis, Volume 43, Issue 3, Page(s) 1174-1178.||摘要:||
We have used LC/ion trap tandem MS analysis to determine saikosaponin-a and -c as target markers in crude 70% methanol extracts from three different species of Bupleuri radix and the 10 most-popular Chinese medicinal preparations containing "Chaihu" (B. radix) without any clean-up. The optimal ionization characteristics were obtained when using positive-ion electrospray ionization (ESI) with 50 mu M sodium acetate as an additive in the mobile phase. We observed good linearity over the range from 0.02 to 2 mu g/ml for saikosaponin-a and from 0.02 to 1 mu g/ml for saikosaponin-c. The intra-day precisions varied between 3.3 and 8.8% for saikosaponin-a and 0.3 and 11.1% for saikosaponin-c. The limits of detection were 0.01 mu g/ml for both markers. The recoveries of saikosaponin-a and -c from the extract of a medicinal preparation sample (Chai-Hu-Ching-Gan-Tang, No. 13 in the table of section Analysis on actual samples) were 97 and 100%, respectively, at a 1 mu g/ml spiking concentration of each marker. The highest concentrations of saikosaponin-a and -c among the three B. radixes were found in B. kaoi Liu Chao & Chuang (10.1 mg/g) and in B. falcatum (3.4 mg/g), respectively. The amounts of these saikosaponins in the 10 Chinese medicinal preparations ranged between 0.11 and 1.22 mg/g for saikosaponin-a and between 0.01 and 0.33 mg/g for saikosaponin-c. (c) 2006 Elsevier B.V. All rights reserved.
|Appears in Collections:||化學系所|
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