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|標題:||The effect of microgrooved and ECM modified surfaces on the alignment of Schwann cells
|作者:||yu, Chen chun
|關鍵字:||microgroove;微米溝槽;Schwann cells;type I collagen;laminin;tissue engineering peripheral nerve repair;許旺細胞;第一型膠原蛋白;層黏連蛋白;組織工程周邊神經修復||出版社:||化學工程學系||摘要:||
In this study, the silicon wafers bearing microgrooved surfaces were designed to study the effects of 3D microstructures on the alignment of rat Schwann cells. The responses of Schwann cells were observed under various sizes of groove width, spacing and depth. By alignment angle assay, the best feature sizes were width/ spacing/ depth= 20/20/1.5 μm. Besides, the extracellular matrix(ECM), type I collagen and laminin, were used to modify the material surfaces. Through the alignment angle analysis, the alignment percentage of laminin-coated surfaces was about 60% and collagen-coated surfaces was about 51%. Laminin was found to have better alignment effects than collagen.
In order to apply the above results to tissue engineering peripheral nerve repair, the microgrooves were transferred to biodegradable poly(D,L-lactide-co-glycolide) films by the method of solvent casting. After laminin modification processes, the combined effects of the physical and chemical signals on the alignment of Schwann cells were assessed. The alignment effect on PLGA surfaces were less evident than on the silicon wafers. The alignment percentage was about 47% on laminin-coated PLGA surfaces.
|Appears in Collections:||化學工程學系所|
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