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標題: Amino acid analysis using disposable copper nanoparticle plated electrodes
作者: Zen, J.M.
Hsu, C.T.
Kumar, A.S.
Lyuu, H.J.
Lin, K.Y.
關鍵字: capillary liquid-chromatography;printed carbon electrodes;amperometric;detection;electrochemical detection;chelation kinetics;peptides;derivatization;fluorescence;resolution;complexes
Project: Analyst
期刊/報告no:: Analyst, Volume 129, Issue 9, Page(s) 841-845.
A disposable copper nanoparticle-plated screen-printed carbon electrode (designated as Cu-n-SPE100-nm) provides a new material for the determination of native amino acids. All 20 underivatized amino acids can be sensitively determined at 0.0 V vs. Ag/AgCl in pH 8 phosphate buffer solution. The precisely controlled copper nanoparticles can boost up the (CuO)-O-II/(Cu2O)-O-I redox signal on the working surface without any prior pretreatment procedure. The formation of a reversible 1:1 (CuO)-O-II-amino acid complex on the Cu-n-SPE100-nm was proposed to play a key role in the reaction mechanism. Stable detection responses were obtained for all amino acids by flow injection analysis with detection limits (S/N=3) that lie in the range of 24 nM-2.7 muM. Selected amino acids from six representative chemical natures were separated by HPLC and detected at the Cu-n-SPE100-nm with promising results.
ISSN: 0003-2654
DOI: 10.1039/b401573h
Appears in Collections:化學系所

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