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標題: A glucose biosensor employing a stable artificial peroxidase based on ruthenium purple anchored cinder
作者: Zen, J.M.
Kumar, A.S.
Chung, C.R.
關鍵字: modified glassy-carbon;prussian blue;modified electrode;hydrogen-peroxide;electrocatalytic oxidation;electrochemical;oxidation;platinum-electrodes;clay;oxidase;sensor
Project: Analytical Chemistry
期刊/報告no:: Analytical Chemistry, Volume 75, Issue 11, Page(s) 2703-2709.
Iron-enriched industrial waste cinder (CFe*) has been recycled for efficient and stable anchoring of Ru(CN)(6)(4-) to the formation of a hybrid ruthenium purple complex. The cinder/ruthenium purple hybrid-modified carbon paste electrode (designated as CPE/CFe*-RP) was worked out for hydrodynamic analysis of H2O2 at a low detecting potential of 0.0 V versus Ag/AgCl in pH 7 ammonium buffer solution. The highly active, selective, and stable electrocatalytic system with a function similar to peroxidase enzyme shows a linear calibration curve up to 0.8 mM H2O2 at a rotation rate of 3600 rpm with slope and detection limit (S/N = 3) of 0.11 muA/muM and 33 nM, respectively. Interference by direct electrochemical oxidation of easily oxidizable substances can be prevented as a result of the low detecting potential of the working system. A glucose biosensor was further constructed by coating with glucose oxidase and Tosflex on the CPE/CFe*-RP (denoted as CPE/CFe*-RP/GOx/Ts). The proposed CPE/CFe*-RP/GOx/Ts with a two-layer configuration, that is, enzyme and protecting layers, exhibits good operational performance in terms of response time, linearity, detection limit, and lifetime.
ISSN: 0003-2700
DOI: 10.1021/ac020542u
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