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標題: Activated Nickel Platform for Electrochemical Sensing of Phosphate
作者: Cheng, W.L.
Sue, J.W.
Chen, W.C.
Chang, J.L.
Zen, J.M.
關鍵字: flow-injection analysis;self-assembled monolayer;liquid-chromatography;pyruvate oxidase;natural-waters;waste-water;electrode;biosensor;system;sensor
Project: Analytical Chemistry
期刊/報告no:: Analytical Chemistry, Volume 82, Issue 3, Page(s) 1157-1161.
We report here a highly selective enzymeless approach for the determination of phosphate (PO(4)(3-)) by flow injection analysis (FIA). In this system, the activation of barrel plated nickel electrode (Ni-BPE) in alkaline media to form a Ni(OH)(2)/NiO(OH) film was found to trigger the adsorption of phosphate at the electrode surface. Based on the suppressed current of the electrocatalytic oxidation of glucose at the activated Ni-BPE in 0.1 M NaOH solution caused by adsorption of phosphate, we develop an FIA detection scheme for the determination of phosphate. Under the optimized conditions of flow rate =300 mu L/min and detection potential =0.55 V vs Ag/AgCl with 25 mu M glucose in 0.1 M NaOH as carrier solution, the calibration curve showed a linear range up to 1 mM. Possible interferences from the coexisting ions were also investigated. The results demonstrated that sensor could be used for the determination of phosphate in the presence of nitrate, chloride, sulfate, acetate, oxalate, carbonate, and some anionic species of toxicological and environmental interest, such as chlorate, chromate, and arsenate ions. The electrode can be effectively regenerated without extra treatment under the hydrodynamic condition. For eight continuous injections of 40 mu M PO(4)(3-), a relative standard deviation of 0.28% was obtained, indicating good reproducibility of the proposed method. ne detection limit (S/N = 3) was calculated as 0.3 mu M.
ISSN: 0003-2700
DOI: 10.1021/ac9025253
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