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|標題:||Dynamic endothelial cell seeding, culture and physical stimulation for poly(carbonate)urethane small-diameter vascular grafts
|關鍵字:||PCU;聚碳酸酯型聚胺酯;ultrasound;laser;dynamic seeding;dynamic culture;vWF;超音波;雷射;動態植覆;動態培養;vWF||出版社:||化學工程學系||摘要:||
The biomedical grade poly(carbonate)urethane synthesized in our lab was used to fabricate the small-diameter vascular grafts by salt-impregnating. The grafts had open-cell structure with a compliance similar to that of native vessels, conformed by SEM and mechanical tests.
To improve the seeding efficiency, an RGD coating protein, was coated on the internal surface of grafts before cell seeding, and the endothelial cells were pretreated stimulated by ultrasound or laser stimulation. It was found that efficiency was not enhanced by ultrasound or laser. However the endothelial cell retention after 3-hr flushing experiment was CBD-RGD. Ultrasound stimulation could increase the ability of endothelial cells to adhere on the internal surface of grafts, and make them more resistant to the shear force of the fluid. It was also shown by SEM and GAG analysis that endothelial cells cultured in the dynamic condition secreted more extracellular matrix to confront with the force of fluid. For endothelial cells cultured in the static condition, the cell activity and functionality might decrease with the increasing times of subculture.
It was found that the optimized culture and processes conditions of vascular grafts for implantation in animal body were: (1)before seeding the endothelial cells were expanded and stimulated by ultrasound for a week; (2)the cells seeded on the microporous PCU+gelatin+RGD grafts by dynamic rotational 0.16rpm seeding; (3)seeded grafts were cultured statically for 72hours and dynamically (perfusion type) for a week.
|Appears in Collections:||化學工程學系所|
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