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|標題:||Evaluation of cell seeding, differentiation and bioreactor culture for scaffolds manufactured by the fused deposition modeling
Cheng, Jia sheng
|關鍵字:||骨先驅細胞;osteoprogenitor cells;ALP活性;精密支架;動態培養;生物反應器;ALP activity;dynamic culture;precision scaffolds;bioreactor||出版社:||化學工程學系||摘要:||
In the first part of this study, the alkaline phosphatase (ALP) activity and calcification of osteoprogenitor cells after being stimulated with the differentiating reagent were measured. The ALP activity was found to increase significantly after ten days and decrease after eighteen days. The control osteoprogenitor cells without the differentiating reagent were not calcified. The cells with the differentiating reagent were calcified after ten days, and more after eighteen days, as demonstrated by the von Kossa stain.
To enhance the cell seeding efficiency in the precision scaffolds, different methods have been compared. By dropping the cell suspensions on the scaffolds, we were able to increase the seeding efficiency to ∼50%. When the precision scaffolds had a concentric circle (i.e. loose interior) structure, the seeding efficiency could reach 66%.
In the three-dimensional cell culture, the scaddolds seeded with osteoblasts were placed in a rotational bioreactor with a speed of 6 rpm. After four weeks, more cell proliferation was observed in PLLA freeze-dried scaffolds. Precision scaffolds, on the other hand, had more extracellular matrix. Based on these results, we have established a cell-precision scaffold-bioreactor model for more in-depth study in the future.
|Appears in Collections:||化學工程學系所|
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