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標題: 利用葡萄聚糖接枝聚乙烯亞胺載體對動物細胞轉染效率之探討
作者: 唐建翔
Hsiang, Tang Chien
關鍵字: 葡萄聚糖;聚乙烯亞胺;基因傳遞;細胞轉染
出版社: 化學工程學系
In this study, dextran of molecular weight 1500 was grafted onto branched polyethylenimine ( PEI ). The dextran-grafted PEI was mixed with plasmid pEGFP-C1 to form complexes to transfect CHO and 231 cells. It was found that the transfection efficiency was enhanced when PEI was prepared by the reaction using low weight ratios of dextran to PEI ( 0.25 and 0.5 ). When EMA-labeled plasmid was used to examine plasmid delivery, it was found that the intracellular amounts of the delivered plasmid within CHO cells became less for using dextran-grafted PEI. The cytotoxicity of the dextran-grafted PEI was slighty reduced when the DNA / polymer complexes were used to transfect CHO cells. As the titration experiments revealed, the buffering capacity of the dextran-grafted PEI was higher than that of the unmodified PEI, and increased as the grafting degree decreased. The increased buffering capacity might cause the enhanced transfection efficiency.

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