Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/34840
標題: A new fabrication process for a microchip electrophoresis device integrated with a three-electrode electrochemical detector
作者: Tsai, D.M.
曾志明
Lin, K.W.
Zen, J.M.
Chen, H.Y.
Hong, R.H.
關鍵字: human urine;microchip electrophoresis;miniaturization;screen-printed;electrode;screen-printed electrode;capillary-electrophoresis;ascorbic-acid;uric-acid;immunoglobulin-g;chip;injection
Project: Electrophoresis
期刊/報告no:: Electrophoresis, Volume 26, Issue 15, Page(s) 3007-3012.
摘要: 
We report here a novel and simple process for the fabrication of a poly(methyl methacrylate) (PMMA)-based microchip electrophoresis device, integrated with a screen-printed three-electrode electrochemical detector that does not require a replicate mold. In this approach, a photoresist layer constitutes both an adhesion layer and side walls of 50 mu m wide and 50 mu m tall microfluidic channels on a screen-printed three-electrode PMMA substrate. Openings were drilled for buffer reservoirs on an additional piece of PMMA, then the final device was bonded in a PMMA/photoresist/PMMA sandwich configuration. This process is inexpensive, less time-consuming, and simpler compared with traditional fabrication methods. The combination of this PMMA-based microchip fabrication together with screen-printed electrode technology holds great promise for the mass production of a single-use micrototal analytical system. Successful determination of uric acid and L-ascorbic acid with the presented system validates its utility. In combination with a suitable electrochemical detector, this device holds much promise for the determination of other analytes in various biological samples for medical and clinical diagnosis.
URI: http://hdl.handle.net/11455/34840
ISSN: 0173-0835
DOI: 10.1002/elps.200500107
Appears in Collections:化學系所

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