Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/35037
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dc.contributor.authorShieh, M.J.en_US
dc.contributor.author葉鎮宇zh_TW
dc.contributor.authorPeng, C.L.en_US
dc.contributor.authorLou, P.J.en_US
dc.contributor.authorChiu, C.H.en_US
dc.contributor.authorTsai, T.Y.en_US
dc.contributor.authorHsu, C.Y.en_US
dc.contributor.authorYeh, C.Y.en_US
dc.contributor.authorLai, P.S.en_US
dc.contributor.author賴秉杉zh_TW
dc.date2008zh_TW
dc.date.accessioned2014-06-06T07:48:41Z-
dc.date.available2014-06-06T07:48:41Z-
dc.identifier.issn0168-3659zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/35037-
dc.description.abstractDevelopment of controllable and non-toxic gene transfection systems is a core issue in gene therapy. Photochemical internalization, an innovative strategy in cytosolic release, provides us with an opportunity to develop a light-inducible gene delivery system. In this study, a novel photochemical internalization (PCI)-mediated gene delivery system was synthesized by surface modification of polyamidoamine (PAMAM) dendrimers via 5,10,15-tri(4-acetamidophenyl)-20-mono(4-carboxyl-phenyl)porphyrin (TAMCPP) conjugated to the generation 4 PAMAM dendrimer (G4). This water-soluble PAMAM-TAMCPP conjugate was characterized for cell viability, phototoxicity, DNA complexation, and in vitro transfection activity. The results show that TAMCPP conjugation did not increase the cytotoxicity of the PAMAM dendrimer below 20 mu M, but significantly induced cell death after suitable irradiation. Under almost non-toxic G4-TAMCPP-mediated PO treatment, the expression of green fluorescent protein determined by flow cytometry could be markedly enhanced in HeLa cells. Therefore, the G4-TAMCPP conjugate had an inducible and effective gene transfection activity, and showed considerable potential as a bimodal biomaterial for PCI-mediated gene therapy. Crown Copyright (C) 2008 Published by Elsevier B.V. All rights reserved.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Controlled Releaseen_US
dc.relation.ispartofseriesJournal of Controlled Release, Volume 129, Issue 3, Page(s) 200-206.en_US
dc.relation.urihttp://dx.doi.org/10.1016/j.jconrel.2008.03.024en_US
dc.subjectpolyamidoamine dendrimeren_US
dc.subjectphotochemical internalizationen_US
dc.subjectcytotoxicityen_US
dc.subjecttransfectionen_US
dc.subjectphotochemical internalizationen_US
dc.subjectpolyamidoamine dendrimersen_US
dc.subjectdrug-deliveryen_US
dc.subjectphotodynamic therapyen_US
dc.subjectcancer-therapyen_US
dc.subjectdna complexesen_US
dc.subjectcellsen_US
dc.subjectvectorsen_US
dc.subjectphotosensitizeren_US
dc.subjectdisruptionen_US
dc.titleNon-toxic phototriggered gene transfection by PAMAM-porphyrin conjugatesen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1016/j.jconrel.2008.03.024zh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.cerifentitytypePublications-
item.fulltextno fulltext-
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