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標題: Capillary electrophoretic analysis of gamma-aminobutyric acid and alanine in tea with in-capillary derivatization and fluorescence detection
作者: Lin, Y.P.
Su, Y.S.
Jen, J.F.
關鍵字: capillary electrophoresis;in-capillary derivatization;GABA;alanine;amino-acids;biogenic-amines;gaba;microdialysis;electrochromatography;chromatography;metabolism;injection;histamine;glutamate
Project: Journal of Agricultural and Food Chemistry
期刊/報告no:: Journal of Agricultural and Food Chemistry, Volume 55, Issue 6, Page(s) 2103-2108.
The aim of this study was to investigate an in-capillary derivatization capillary electrophoresis (CE) technique that was performed to determine the concentration of gamma-aminobutyric acid (GABA) and alanine (Ala) in tea after being derivatized with o-phthaldialdehyde/2-mercaptoethanol (OPA/2-ME) to form fluorescence-labeled products. The conditions of labeled derivatization and CE separation were optimized and then applied to real sample analysis. The labeled derivatization with 20 mM OPA and 26.67 mM 2-ME (mol ratio = 0.75) at pH 10 offered the most sensitive detection, and the separation with 30 mM sodium tetraborate buffer (pH 10.0) under 21 kV achieved good selectivity within 14 min. The detections were linear in the range of 0.05-5 mu M with correlation coefficients (R-2) of 0.9995 and 0.9964 and with detection limits of 0.004 and 0.02 mu M for GABA and Ala, respectively. The recoveries were 94.22% (3.58% RSD) and 93.54% (6.46% RSD) for five determinations of GABA and Ala, respectively. This method is a fast, convenient, sensitive, and eco-friendly way to determine the GABA and Ala in tea samples from different manufacturing processes.
ISSN: 0021-8561
DOI: 10.1021/jf062996o
Appears in Collections:化學系所

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