Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/35054
DC FieldValueLanguage
dc.contributor.authorSu, Y.S.en_US
dc.contributor.author鄭政峰zh_TW
dc.contributor.authorLin, Y.P.en_US
dc.contributor.authorCheng, F.C.en_US
dc.contributor.authorJen, J.F.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-06T07:48:42Z-
dc.date.available2014-06-06T07:48:42Z-
dc.identifier.issn0021-8561zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/35054-
dc.description.abstractAn in-capillary derivatization and stacking capillary electrophoresis (CE) technique has been applied to redeem the detection of dilute analytes in the analysis of gamma-aminobutyric acid (GABA) and alanine (Ala) in tea samples. Extracts from samples were diluted to eliminate matrix interference before introduction into the CE system. GABA and Ala in the diluted sample zone were derivatized with o-phthaldialdehydp-/2-mercaptoethanol (OPA/2-ME) to form fluorescence-labeled products in the stacking process, and the labeled derivatives were then enriched by online stacking. Optimal conditions for the stacking, such as the concentration of the background buffer solution, the matrix of the sample zone (sample solution), and the volume of the sample injection, were investigated and then applied to real sample analysis. Under optimum conditions, the detections were linear in the range of 5.0 nM-2.5 mu M with the square of correlation coefficients (R(2)) of 0.9995 and 0.9992 for GABA and Ala, respectively. Detection limits were found to be 0.7 and 0.8 nM for GABA and Ala, respectively. Tea samples were analyzed with recoveries between 92.33 and 97.87% and between 94.36 and 96.46% for GABA and Ala, respectively. This method is a rapid, convenient, and sensitive process for determining GABA and Ala in complicated matrix samples such as tea samples.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Agricultural and Food Chemistryen_US
dc.relation.ispartofseriesJournal of Agricultural and Food Chemistry, Volume 58, Issue 1, Page(s) 120-126.en_US
dc.relation.urihttp://dx.doi.org/10.1021/jf902958uen_US
dc.subjectgamma-Aminobutyric aciden_US
dc.subjectcapillary electrophoresisen_US
dc.subjectderivatizationen_US
dc.subjectin-capillary stackingen_US
dc.subjectmatrix interferenceen_US
dc.subjectinduced fluorescence detectionen_US
dc.subjectbiogenic-aminesen_US
dc.subjectpseudo-isotachophoresisen_US
dc.subjectonline preconcentrationen_US
dc.subjectzone-electrophoresisen_US
dc.subjectinjectionen_US
dc.subjectceen_US
dc.subjectelectrochromatographyen_US
dc.subjectchromatographyen_US
dc.subjectsystemen_US
dc.titleIn-Capillary Derivatization and Stacking Electrophoretic Analysis of gamma-Aminobutyric Acid and Alanine in Tea Samples To Redeem the Detection after Dilution To Decrease Matrix Interferenceen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1021/jf902958uzh_TW
item.grantfulltextnone-
item.openairetypeJournal Article-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
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