Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/35079
DC FieldValueLanguage
dc.contributor.authorChen, S.J.en_US
dc.contributor.author鄭政峰zh_TW
dc.contributor.authorCheng, F.C.en_US
dc.contributor.authorJen, J.F.en_US
dc.date2010zh_TW
dc.date.accessioned2014-06-06T07:48:44Z-
dc.date.available2014-06-06T07:48:44Z-
dc.identifier.issn1615-9306zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/35079-
dc.description.abstractA microdialysis sampling (MDS) on-line SPE (MDS/SPE) has been applied to redeem the detection after dilution to decrease matrix interference in the analysis of ketamine (K) and its two main metabolites, norketamine (NK) and dehydronorketamine (DHNK) in urine by HPLC. After being filtrated, diluted and adjusting the pH, K and its metabolites in the diluted sample solution were collected through MDS and then trapped on an on-line SPE for HPLC analysis. The optimal conditions for MDS/SPE were investigated and then applied to real sample analysis. Experimental results indicated that the MDS/SPE by using regenerated cellulose hollow fiber (8-cm length) and 1 mM sulfuric acid as the perfusate at 20 mu L/min flow-rate to collect analytes from 100-fold diluted urine sample (20 mL at pH 6.0), and then having been trapped in octadecyl-modified silica phase SPE for 30 min, offered the optimum efficiency. The concentration levels of 41, 42 and 28% (m/m) for K, NK and DHNK, respectively, in urine were redeemed for determination. The detection limits were 0.38, 0.33 and 0.34 ng/mL (in 100-fold diluted sample) for K, NK and DHNK, respectively. The method provides a very simple, inexpensive and eco-friendly procedure to determine K, NK and DHNK in urine.en_US
dc.language.isoen_USzh_TW
dc.relationJournal of Separation Scienceen_US
dc.relation.ispartofseriesJournal of Separation Science, Volume 33, Issue 13, Page(s) 2010-2016.en_US
dc.relation.urihttp://dx.doi.org/10.1002/jssc.201000002en_US
dc.subjectKetamineen_US
dc.subjectMatrix interferenceen_US
dc.subjectMicrodialysis samplingen_US
dc.subjectSPEen_US
dc.subjectUrineen_US
dc.subjectchromatography-mass spectrometryen_US
dc.subjectin-vitro microdialysisen_US
dc.subjectliquid-chromatographyen_US
dc.subjectgc-msen_US
dc.subjectchemical derivatizationen_US
dc.subjectnorketamineen_US
dc.subjectsamplesen_US
dc.subjectplasmaen_US
dc.subjectdehydronorketamineen_US
dc.subjectdrugsen_US
dc.titleOn-line microdialysis coupled solid-phase extraction to decrease matrix interference in the HPLC analysis of urinary ketamine and its metabolitesen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1002/jssc.201000002zh_TW
item.cerifentitytypePublications-
item.grantfulltextnone-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.openairetypeJournal Article-
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