Please use this identifier to cite or link to this item: http://hdl.handle.net/11455/35124
DC FieldValueLanguage
dc.contributor.authorYang, J.en_US
dc.contributor.author顏宏真zh_TW
dc.contributor.authorYen, H.E.en_US
dc.contributor.author楊吉斯zh_TW
dc.date2002zh_TW
dc.date.accessioned2014-06-06T07:48:49Z-
dc.date.available2014-06-06T07:48:49Z-
dc.identifier.issn0032-0889zh_TW
dc.identifier.urihttp://hdl.handle.net/11455/35124-
dc.description.abstractA technique based on Fourier transform infrared (FT-IR) spectrometry was developed to detect the corresponding changes in chemical composition associated with the rapid changes in sodium and water content in 200 mm NaCl-stressed halophyte ice plants (Mesembryanthemum crystallinum). The changes in glycophyte Arabidopsis stressed with 50 mm NaCl were also examined for comparison. The obtained IR spectra were further processed by deconvolution and curve fitting to examine the chemical nature of the responding sources in the leaves. Using three stages of ice plant leaves, absorption bands corresponding to carbohydrates, cell wall pectin, and proteins were identified, with distinct IR spectra representing each developmental stage. Within 48 h of mild salt stress, the absorption band intensities in the fingerprint region increased continuously in both plants, suggesting that the carbon assimilation was not affected at the early stage of stress. The intensities of ester and amide I absorption bands decreased slightly in Arabidopsis but increased in ice plant, suggesting that the cell expansion and protein synthesis ceased in Arabidopsis but continued in ice plant. In both plants, the shift in amide I absorption band was observed hourly after salt stress, indicating a rapid conformational change of cellular proteins. Analyses of the ratio between major and minor amide I absorption band revealed that ice plant was able to maintain a higher-ordered form of proteins under stress. Furthermore, the changes in protein conformation showed a positive correlation to the leaf sodium contents in ice plant, but not in Arabidopsis.en_US
dc.language.isoen_USzh_TW
dc.relationPlant Physiologyen_US
dc.relation.ispartofseriesPlant Physiology, Volume 130, Issue 2, Page(s) 1032-1042.en_US
dc.relation.urihttp://dx.doi.org/10.1104/pp.004325en_US
dc.subjectprotein secondary structureen_US
dc.subjecthalophyte mesembryanthemum-crystallinumen_US
dc.subjectcrassulacean acid metabolismen_US
dc.subjectatr wave-guidesen_US
dc.subjectfacultative halophyteen_US
dc.subjectdesiccation toleranceen_US
dc.subjectabscisic-aciden_US
dc.subjecthigh salinityen_US
dc.subjectcell-wallsen_US
dc.subjectexpressionen_US
dc.titleEarly salt stress effects on the changes in chemical composition in leaves of ice plant and Arabidopsis. A Fourier transform infrared spectroscopy studyen_US
dc.typeJournal Articlezh_TW
dc.identifier.doi10.1104/pp.004325zh_TW
item.cerifentitytypePublications-
item.openairecristypehttp://purl.org/coar/resource_type/c_18cf-
item.languageiso639-1en_US-
item.fulltextno fulltext-
item.grantfulltextnone-
item.openairetypeJournal Article-
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