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|標題:||Multiple screening of urolithic organic acids with copper nanoparticle-plated electrode: Potential assessment of urolithic risks||作者:||Yang, C.W.
|關鍵字:||Urolithiasis;Creatinine;Organic acids;Electrochemical;Nanoparticle-plated electrode;performance liquid-chromatography;uric-acid;capillary-electrophoresis;electrochemical detection;calcium-oxalate;canine uroliths;stone;formers;renal-disease;nephrolithiasis;creatinine||Project:||Analytical Biochemistry||期刊/報告no：:||Analytical Biochemistry, Volume 395, Issue 2, Page(s) 224-230.||摘要:||
There is yet to be a reliable prediction of urolithiasis. To facilitate early diagnosis, a simple and rapid high performance liquid chromatography method with electrochemical detection using disposable copper-nanoparticle-plated electrodes (Cu(n)-SPE) was developed for multiple detection of creatinine and 4 urolithic organic acids. A total of 206 normal and urolithic human and canine urines and urolith samples were collected for direct analysis of creatinine, cystine, uric acid, oxalic acid, and citric acid without sample cleanup and derivatization processes. Urinary organic acids were separated in 11 min and were devoid of ascorbic acid interference. The detection limits (S/N > 3) were at the nanomolar level with linear dynamic ranges spanning 2-3 orders of magnitude. Recoveries in urine ranged from 99.5% for creatinine to 86.5% for citric acid. The analytical variations (RSD) were less than 6.2% in phosphate buffer and 7.7% in urine. Important differences in organic acid levels/profiles between animal species and among normal and urolithic urines/urolith were unveiled and corresponded well (70-90%) with the urolithic risk in a retrospective assessment. The simplicity and reproducibility of this method using disposable Cu(n)-SPE has made routine urine analysis possible and can be of great clinical and diagnostic potential in the screening of urolithiasis and abnormal states related to excess secretion of organic acids and amino acids in humans and animals. (C) 2009 Elsevier Inc. All rights reserved.
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